For preparation of buffers in the pH range of 6.2 - 7.8 (25 °C)Imidazole is used for the elution of His-tagged recombinant proteins in immobilized metal-affinity chromatography (IMAC), as a chelator for the binding of various divalent cations and in reverse staining of SDS-PAGE protein gels. For preparation of buffers in the pH range of 6.2 - 7.8 (25 °C)Imidazole is used for the elution of His-tagged recombinant proteins in immobilized metal-affinity chromatography (IMAC), as a chelator for the binding of various divalent cations and in reverse staining of SDS-PAGE protein gels.