USP. Tris(hydroxymethyl)aminomethane (Tris) buffer quality for all standard applications, suitable for preparative purposes and for chromatography.With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5. It is used as a component in buffer solutions in numerous applications: • Assays in molecular biology and cytology • Extraction of proteins or nucleic acids from cells • In situ hybridisation procedures • Sample and running buffers for SDS-PAGE • TAE- and TBE buffers for agarose gel electrophoresis • Transfer buffer for Western Blotting • Washing buffers for immunoassays USP. Tris(hydroxymethyl)aminomethane (Tris) buffer quality for all standard applications, suitable for preparative purposes and for chromatography.With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5. It is used as a component in buffer solutions in numerous applications: • Assays in molecular biology and cytology • Extraction of proteins or nucleic acids from cells • In situ hybridisation procedures • Sample and running buffers for SDS-PAGE • TAE- and TBE buffers for agarose gel electrophoresis • Transfer buffer for Western Blotting • Washing buffers for immunoassays