ROTI ® Lux OneStep RT-qPCR Master Mixes are suitable for all common types of probe-based assays, for probe-based genotyping using multiplexing and for the analysis of gene expression in quantitative real-time PCR. All components for cDNA synthesis are already included in the mix, so the isolated RNA can be used directly as starting material. The qPCR Master Mixes contain an optimised DNA polymerase that enables ultra-fast runs in less than 20 minutes. Due to the high sensitivity, even the smallest amounts of cDNA (≤1pg) can be detected. The Master Mixes are also characterised by a particularly high temperature stability, which enables pipetting at room temperature and which means that they remain stable even after several Freeze and Thaw cycles (≥10x). Properties: cDNA synthesis and qPCR in one reaction Excellent sensitivity (<0.1 pg RNA per reaction) High temperature stability Ultra-fast qPCR in <35 min Easy handling due to blue staining Applications: Probe-based qPCR analyses (e. g. TaqMan ® ) Multiplexing Gene expression analyses Genotyping ROTI ® Lux OneStep RT-qPCR Master Mix 2x conc., ready-to-use , low ROX Master mix with low concentration (100 nM) of the reference dye ROX (6-Carboxy-X-rhodamine) Suitable for: Quantstudio™ Cycler (Thermofisher) qTower (Analytik Jena) Mx3000P ® qPCR Systeme (Agilent) Rotor-Gene™ (Qiagen) Not a medical device / Not an IVD product