The innuDRY qPCR MasterMix Probe is a novel, storage-stable real-time PCR Master Mix for fluorescence detection using different probe technologies (e.g. hydrolysis, FRET or rehybridization probes, and many more). The mix contains all components for a successful real-time PCR. Besides high-quality dNTP’s and a perfect combination of qPCR enhancers, the hot start polymerase avoids unspecific side products selectively. Additionally, the lyophilized 2-times formulation offers high storage stability at +4 °C and an environmentally friendly delivery at room temperature. After dissolving by using the Resuspension Buffer only the primer, probes and template need to be added to the master mix and filled up to the final volume by using molecular-biology-grade water. The established handling makes the daily use easy and secure. Real-time results are highly reproducible and ideal in terms of efficiency and slope. The innuDRY qPCR MasterMix Probe is a novel, storage-stable real-time PCR Master Mix for fluorescence detection using different probe technologies (e.g. hydrolysis, FRET or rehybridization probes, and many more). The mix contains all components for a successful real-time PCR. Besides high-quality dNTP’s and a perfect combination of qPCR enhancers, the hot start polymerase avoids unspecific side products selectively. Additionally, the lyophilized 2-times formulation offers high storage stability at +4 °C and an environmentally friendly delivery at room temperature. After dissolving by using the Resuspension Buffer only the primer, probes and template need to be added to the master mix and filled up to the final volume by using molecular-biology-grade water. The established handling makes the daily use easy and secure. Real-time results are highly reproducible and ideal in terms of efficiency and slope.