Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol Mechanism Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl. Reduce exposure to toxic chemicals Prepared from phenol of highest purity Packed under argon for maximum stability Successfully tried and tested in many research laboratories ROTI ® Phenol/Chloroform/ Isoamyl alcohol ready-to-use, for the extraction of nucleic acids ROTI ® Phenol/Chloroform/Isoamyl alcohol is re-distilled phenol, equilibrated in TE buffer, premixed with high purity chloroform and isoamyl alcohol. ROTI ® Phenol/Chloroform/Isoamyl alcohol has proven itself in many research laboratories for the isolation of nucleic acids and is an ideal complement to pure ROTI ® Phenol. Mechanism Addition of 1 volume ROTI ® Phenol/Chloroform/Isoamyl alcohol and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. Following centrifugation, the nucleic acid containing solution may be retrieved as upper phase. Draw solution from lower phase. Do not shake before use!