ROTI ® ZOL RNA ready-to-use, for molecular biology ROTI ® ZOL RNA is a ready-to-use, green-coloured solution for isolating total RNA from biological samples such as cells or tissue. ROTI ® ZOL RNA is based on the phenolic isolation procedure known from Chomczynski and Sacchi ( Anal. Biochem. (1987) 162:156-9). Due to the advanced and optimized formulation, ROTI ® ZOL RNA results in very high recovery rates of highly pure total RNA. Application is very convenient and preparation of several samples in parallel is finished in less than one hour. Additionally, the green colour significantly simplifies the separation of the RNA containing aqueous phase, the DNA containig interphase, and the protein containing phenolic phase during the process. Easy and very rapid two-step RNA isolation High recovery rates of intact, very pure total RNA Applicable on a broad range of tissues and on cell types The purified RNA is ready for use in standard downstream applications such as RT-PCR Typical RNA yield from 5x10 6 cells: 30-35 μg Typical purity of RNA prepared from 5x10 6 cells: 2,08 (A 260 /A 280 ) Figures 1 and 2: Isolation of totalRNA by ROTI ® Zol RNA. Left: Tube after centrifugation. A: Aqueous phase - RNA containig, P: Phenolic phase - protein containing. DNA accumulates in the A/P interphase. Right: Typical gel of total RNA isolated by ROTI ® Zol RNA (extracted from 5x10 6 HL-60 cells).