Recommended for applications requiring controlled solvent behavior, such as amino acids HILIC applications, highly polar analytes, hydrophilic compounds, nucleosides, oligonucleotides, organic polar acids and bases, peptides, polar natural products, water-soluble vitamins, and other similar uses, the Hplc Column Nucleodur® Hilic, 5 μm, 250 mm, 4 mm offers a solution. This analytical separation column features a hydrophilic interaction chromatography (HILIC) phase based on high purity NUCLEODUR® silica gel, providing an effective strategy for separating polar analytes in HPLC. It boasts a specific surface area of 340 m²/g, ensuring efficient interactions and retention mechanisms through its ionic and hydrophilic properties. With a pH

• Category: Separation columns for HPLC
• Column volume: 3.15 ml
• Endcapped: no
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Application Database (Macherey-Nagel): HPLC
• Pressure stability: 600 bar
• Pore size: 110 Å
• Column type: HPLC column
• Eluent in column: Acetonitrile - Water
• pH stability: 2,0-8,5
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Particle size: 5 µm
• Temperature stability: 60 ° C
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Specific surface according to BET: 340 m²/g
• Column shape: Analytical separation column
• Pore volume: 0.9 ml/g
• Phase: HILIC
• L x Ø internal (column): 250 x 4 mm
• Particle type: Fully porous particles (FPP)
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• USP listing: Not available or proprietary