Crafted to support technical production environments, the Hplc Column Nucleodur® C 4 Ec, 100 MM, 4.6 MM offers an ideal solution for separating biomolecules such as proteins and peptides. This analytical separation column features a medium density monomeric butyl (C 4 ) wide-pore modification, ensuring lower retention for hydrophobic compounds compared to other common modifications like C 18 or C 8. With a pressure stability of 450 bar and temperature stability up to 60°C, this column is designed to handle demanding conditions while maintaining its performance. The column comes equipped with fully porous particles (FPP) and a specific surface area of 100 m²/g, making it suitable for use with acetonitrile-water eluents. Delivering a column volume of 1.6

• Category: Separation columns for HPLC
• Eluent in column: Acetonitrile - Water
• USP listing: L26
• L x Ø internal (column): 100 x 4.6 mm
• Similar phases: Jupiter ® C 4 ACE ® C 4
• Pressure stability: 450 bar
• Pore size: 300 Å
• Particle size: 5 µm
• Column shape: Analytical separation column
• Phase: C 4 ec
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• Separation principle: Reversed phase (RP)
• Column volume: 1.65 ml
• Pore volume: 0.9 ml/g
• Endcapped: yes
• Surface chemistry: Medium density monomeric butyl (C 4 ) wide-pore modification
• Particle type: Fully porous particles (FPP)
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions
• Specific surface according to BET: 100 m²/g
• Temperature stability: 60 ° C
• Column type: HPLC column
• pH stability: 1,0-9,0