Configured for stable performance in synthesis settings, the Hplc Column Nucleodur® Hilic, 5 μm, 125 mm, 2 mm offers a unique solution for the separation of highly polar analytes such as amino acids, peptides, water-soluble vitamins, and organic polar acids and bases. This analytical separation column features a hydrophilic interaction chromatography (HILIC) phase, based on high purity NUCLEODUR® silica gel, which provides excellent separation efficiency for these challenging compounds. With a particle size of 5 μm, this column delivers optimal pressure stability up to 600 bar and temperature stability up to 60°C, ensuring reliable results even under demanding conditions. Its specific surface area of 340 m²/g further enhances its ability to separate complex mixtures effectively.

• Category: Separation columns for HPLC
• Pore volume: 0.9 ml/g
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• pH stability: 2,0-8,5
• Temperature stability: 60 ° C
• Column type: HPLC column
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Pressure stability: 600 bar
• Particle type: Fully porous particles (FPP)
• Eluent in column: Acetonitrile - Water
• Application Database (Macherey-Nagel): HPLC
• Column volume: 0.37 ml
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Specific surface according to BET: 340 m²/g
• Pore size: 110 Å
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Endcapped: no
• L x Ø internal (column): 125 x 2 mm
• Particle size: 5 µm
• Column shape: Analytical separation column
• Phase: HILIC
• USP listing: Not available or proprietary