Order number: 35PN.1
Part number: AC100007037
Designed for professional use, the Hplc Column Nucleodur ® Hilic, 1.8 μm, 100 mm, 4.6 mm is a UHPLC column specifically designed for separating highly polar analytes such as amino acids, peptides, water-soluble vitamins, and other hydrophilic compounds. This column utilizes the hydrophilic interaction chromatography (HILIC) principle, featuring an ammonium – sulfonic acid (zwitterionic) modified surface that provides ionic and hydrophilic interactions for efficient retention of polar analytes. With a particle size of 1.8 μm, this column offers excellent pressure stability up to 900 bar and temperature stability up to 60°C. The column's specific surface area of 340 m²/g ensures optimal separation
| Name | Value | |
|---|---|---|
| Phase | : | HILIC |
| Endcapped | : | no |
| Pore size | : | 110 Å |
| Column type | : | UHPLC column |
| Pore volume | : | 0.9 ml/g |
| USP listing | : | Not available or proprietary |
| Column shape | : | Analytical separation column |
| pH stability | : | 2,0-8,5 |
| Column volume | : | 1.65 ml |
| Particle size | : | 1.8 µm |
| Particle type | : | Fully porous particles (FPP) |
| Eluent in column | : | Acetonitrile - Water |
| Surface chemistry | : | Ammonium – sulfonic acid (zwitterionic) modification |
| Pressure stability | : | 900 bar |
| Separation principle | : | Hydrophilic Interaction Chromatography (HILIC) |
| Temperature stability | : | 60 ° C |
| L x Ø internal (column) | : | 100 x 4.6 mm |
| Recommended application(s) | : | Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins |
| Specific surface according to BET | : | 340 m²/g |
| Interactions / retention mechanism | : | ionic / hydrophilic and electrostatic |
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK