Technical Advantage:  Effective against Gram-positive and -negative bacteria Puromycin Dihydrochloride is a nucleoside antibiotic and protein synthesis inhibitor.  By acting as an analog of the 3'-terminal end of aminoacyl-tRNA, it prevents growth of bacteria, protozoa, algae, and mammalian cells by causing premature protein termination.  The resistance gene (puromycin acetyltransferase) gives very effective protection.  It is effective against Gram-negative and Gram-positive bacteria. Technical Advantage:  Effective against Gram-positive and -negative bacteria Puromycin Dihydrochloride is a nucleoside antibiotic and protein synthesis inhibitor.  By acting as an analog of the 3'-terminal end of aminoacyl-tRNA, it prevents growth of bacteria, protozoa, algae, and mammalian cells by causing premature protein termination.  The resistance gene (puromycin acetyltransferase) gives very effective protection.  It is effective against Gram-negative and Gram-positive bacteria.