[+] 25 mM HEPES,[-] ß-thioglycerol, ß-mercaptoethanol, L-glutamine Iscove's Modification of DMEM (IMDM) is highly enriched synthetic medium optimized for rapidly proliferating cultures of high cell density. A modified form of Dulbecco's Modified Eagle Medium (DMEM), Iscove's contains additional amino acids, vitamins, selenium and HEPES buffer, and is formulated with potassium nitrate in place of ferric nitrate. The addition of albumin and transferrin may be required for certain cell types. [+] 25 mM HEPES,[-] ß-thioglycerol, ß-mercaptoethanol, L-glutamine Iscove's Modification of DMEM (IMDM) is highly enriched synthetic medium optimized for rapidly proliferating cultures of high cell density. A modified form of Dulbecco's Modified Eagle Medium (DMEM), Iscove's contains additional amino acids, vitamins, selenium and HEPES buffer, and is formulated with potassium nitrate in place of ferric nitrate. The addition of albumin and transferrin may be required for certain cell types.