ROTI ® Methylene blue staining concentrate 200x conc. ROTI ® Methylene blue staining concentrate was specially developed for sensitive staining of DNA in agarose gels. Sensitivity reaches up to 10 ng per band. Staining can be conducted in a simple staining bowl, a transilluminator is not required. Highly reversible alternative to ethidium bromide High sensivity (in minimum 10 ng/band) No transilluminator required Solution can be used repeatedly Sufficient for more than 50 mini gels Mechanism ROTI ® Methylene blue staining concentrate binds to the negativly charged DNA backbone by salt bridges formed through quaternary ammonia complexes. The staining is very easy to remove by washing or replacement by salts. Directions for use For staining DNA agarose gels, DNA polyacrylamide gels, for reversible staining of Southern- and Northern-Blots. Staining can be performed in a simple staining dish, a transilluminator is not required. The diluted staining solution can be used several times.