The innuPREP cell-free microRNA Kit has been designed for very fast and efficient isolation of cell-free microRNAs from exosomes and other microvesicles. This kit can be combined with the innuPREP PME Exosome Enrichment Kit, ultracentrifugation, filtration and similar methods. The innuPREP cell-free microRNA Kit can also be used for isolation of microRNA from unprocessed samples, exosome-depleted supernatant obtained after exosome separation by above-mentioned techniques or from samples selected by size exclusion chromatography. The extraction procedure is based on a new kind of chemistry, which combines a fast lysis step with subsequent efficient binding of microRNA to a spin filter surface, followed by washing of the bound microRNA and finally elution of the microRNA. Recovery rate and quality of the eluted microRNA are excellent. The innuPREP cell-free microRNA Kit has been designed for very fast and efficient isolation of cell-free microRNAs from exosomes and other microvesicles. This kit can be combined with the innuPREP PME Exosome Enrichment Kit, ultracentrifugation, filtration and similar methods. The innuPREP cell-free microRNA Kit can also be used for isolation of microRNA from unprocessed samples, exosome-depleted supernatant obtained after exosome separation by above-mentioned techniques or from samples selected by size exclusion chromatography. The extraction procedure is based on a new kind of chemistry, which combines a fast lysis step with subsequent efficient binding of microRNA to a spin filter surface, followed by washing of the bound microRNA and finally elution of the microRNA. Recovery rate and quality of the eluted microRNA are excellent.