The innuPREP FFPE total RNA Kit allows the extraction of total RNA with an excellent recovery of microRNAs from FFPE (formalin-fixed, paraffin-embedded) tissue samples. The kit is suitable for starting amounts of up to 50 mg. Based on a new kind of chemistry the kit enables the isolation of RNA from FFPE samples without a deparaffinization step and thereby eliminates the use of toxic and hazardous components like octane or xylene. After only 1.5 h containing lysis, binding, washing and elution the whole extraction routine is finished. Thus the protocol allows the extraction of RNA with high quality, ideal integrity and excellent formaldehyde- RNA de-crosslinking. The isolated RNA including microRNAs is directly suitable for reverse transcription, amplification reaction and other amplification-based downstream applications. The innuPREP FFPE total RNA Kit allows the extraction of total RNA with an excellent recovery of microRNAs from FFPE (formalin-fixed, paraffin-embedded) tissue samples. The kit is suitable for starting amounts of up to 50 mg. Based on a new kind of chemistry the kit enables the isolation of RNA from FFPE samples without a deparaffinization step and thereby eliminates the use of toxic and hazardous components like octane or xylene. After only 1.5 h containing lysis, binding, washing and elution the whole extraction routine is finished. Thus the protocol allows the extraction of RNA with high quality, ideal integrity and excellent formaldehyde- RNA de-crosslinking. The isolated RNA including microRNAs is directly suitable for reverse transcription, amplification reaction and other amplification-based downstream applications.