High optical quality, glass bottom, black microplates are ideal for performing high content cell-based assays using imaging systems. The glass bottom provides a flat and optically clear surface that reduces autofocus time, increases throughput, and is ideal for cell growth. High optical quality, glass bottom, black microplates are ideal for performing high content cell-based assays using imaging systems. The glass bottom provides a flat and optically clear surface that reduces autofocus time, increases throughput, and is ideal for cell growth.