Corning PureCoat Amine (positively charged) and Carboxyl (negatively charged) surfaces provide improved cell attachment, faster cell proliferation, and enhanced recovery post-thaw over standard TC surfaces. These surfaces function with a broad range of primary, transfected, transformed, and fastidious cell types, and have demonstrated utility in serum-reduced or serum-free conditions. Corning PureCoat Amine (positively charged) and Carboxyl (negatively charged) surfaces provide improved cell attachment, faster cell proliferation, and enhanced recovery post-thaw over standard TC surfaces. These surfaces function with a broad range of primary, transfected, transformed, and fastidious cell types, and have demonstrated utility in serum-reduced or serum-free conditions.