3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate or CHAPS is a mild, zwitterionic sulfobetaine detergent, which is non-denaturing to membrane proteins, can solubilize proteins, and disaggregate protein-protein interactions (1 - 5).Due to its zwitterionic nature, CHAPS does not exhibit a net charge between pH 2 to 12, making it also useful in ion exchange chromatography and isoelectric focusing. CHAPS is as well suitable for enzyme immunoassay (6) and as a component of lysis buffer for tissue homogenization, in denaturing buffer in enzyme characterization and of 2D-sample buffer in 2D electrophoresis.Its small micellar molecular weight and high critical micellar concentration allowing it to be removed from samples by dialysis.CMC (25 °C) 4.2 - 6.5 mM, Na (25 °C) 9 - 10 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate or CHAPS is a mild, zwitterionic sulfobetaine detergent, which is non-denaturing to membrane proteins, can solubilize proteins, and disaggregate protein-protein interactions (1 - 5).Due to its zwitterionic nature, CHAPS does not exhibit a net charge between pH 2 to 12, making it also useful in ion exchange chromatography and isoelectric focusing. CHAPS is as well suitable for enzyme immunoassay (6) and as a component of lysis buffer for tissue homogenization, in denaturing buffer in enzyme characterization and of 2D-sample buffer in 2D electrophoresis.Its small micellar molecular weight and high critical micellar concentration allowing it to be removed from samples by dialysis.CMC (25 °C) 4.2 - 6.5 mM, Na (25 °C) 9 - 10