Ethidium bromide Ethidium bromide is a strong mutagenic and toxic substance. For your own safety, always wear gloves and respiratory protection to prevent contamination when working with this substance. After use, dispose of solutions correctly. They may not be discarded into the environment. The following procedure is recommended (acc. to INSERM, Dossier prevention, Number 2, 2nd Ed., September 88): 1. Collect and dispose of as hazardous waste. 2. Break down EtBr with potassium permanganate and HCI (P. Quillardet, M. Hofnung, Trends Genet . 4,89 [1988]). 3. Break down EtBr with hypophosphoric acid and sodium nitrite (Lunn and Sansone; Analyt. Biochem. 162, 453-458 [1987]). 4. Absorption of EtBr with Amberlite ® XAD-16 (Lunn and Sansone) or with activated carbon (Bensaude, O. Method, Trends Genet. 4, 89-90 [1988]) from the solution. We recomment disposure by SEKUROKA ® Decon-Bags (Art. No. T856) Ethidium bromide ≥98 % Fluorescent dye with specific DNA binding capacity. Intercalates between base pair stacks of dsDNA, causing weaker staining of ssDNA or RNA. For staining of DNA/RNA in gels and for counterstaining of nuclei in histological and cytological analyses. Wavelength Excitation maximum: 518 nm Emission maximum: 605 nm