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Methanol ≥99,9 %, Blotting Grade Methanol of very high purity, tested for absence of heavy metals, sulphates, and organic impurities. Particularly suitable for Western blotting.
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Glycine ≥99 %, Blotting Grade Glycin of very high purity, tested for absence of heavy metals, ammonia, and hydrolysable substances. Well soluble and particularly suitable for Western blotting.
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Ponceau S (C.I. 27195) for histology and electrophoresis Directions for use Staining of NC membranes: 0.2-2 % Ponceau S in 3 % trichloroacetic acid (Art. No. 8789.1) + 3 % sulphosalicylic acid (Art. No. 4119.1), incubate for 1-5 mins. at room temperature. Enhance contrast by incubation in water acidified by a few drops acetic acid. Staining of PVDF membranes: 0.1-1 % Ponceau S in 10 % acetic acid (Art. No. 6755.1), incubate 1-5 mins. at room temperature. Suitable for rapid control of transfer in Western blot, PVDF membrane should not be completely dried before immunodetection. Prior to Western blot analysis decolourise in PBS (Art. No. 1058.1) or TBS (Art. No. 1060) for 10-20 mins. at room temperature.
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TRIS ≥99,9 %, Blotting Grade Tris of very high purity, tested for absence of heavy metals and protease. Particularly suitable for Western blotting and protein immunodetection.
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Ponceau S (C.I. 27195) for histology and electrophoresis Directions for use Staining of NC membranes: 0.2-2 % Ponceau S in 3 % trichloroacetic acid (Art. No. 8789.1) + 3 % sulphosalicylic acid (Art. No. 4119.1), incubate for 1-5 mins. at room temperature. Enhance contrast by incubation in water acidified by a few drops acetic acid. Staining of PVDF membranes: 0.1-1 % Ponceau S in 10 % acetic acid (Art. No. 6755.1), incubate 1-5 mins. at room temperature. Suitable for rapid control of transfer in Western blot, PVDF membrane should not be completely dried before immunodetection. Prior to Western blot analysis decolourise in PBS (Art. No. 1058.1) or TBS (Art. No. 1060) for 10-20 mins. at room temperature.
Product Detail
Methanol ≥99,9 %, Blotting Grade Methanol of very high purity, tested for absence of heavy metals, sulphates, and organic impurities. Particularly suitable for Western blotting.
Product Detail
TRIS ≥99,9 %, Blotting Grade Tris of very high purity, tested for absence of heavy metals and protease. Particularly suitable for Western blotting and protein immunodetection.
Product Detail
TRIS ≥99,9 %, Blotting Grade Tris of very high purity, tested for absence of heavy metals and protease. Particularly suitable for Western blotting and protein immunodetection.
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Discontinuous buffer system for optimum transfer in the semi-dry blotter. Optimised for semi dry blotting Superior blotting results for peptides and proteins of every size Special formulation with reduced acid formation - for optimal electrode protection In the semi-dry blotting system, both a continuous buffer system (identical buffer at anode and cathode) and a discontinuous buffer system (different buffer at anode and cathode) can be used. The transfer performance of discontinuous systems is, however, generally higher, since the two buffers are designed separately according to the needs of the two electrodes. The discontinuous buffer system ROTI ® Blot 1 consists of two different buffers, the anode buffer A (pH 7.8 ±0.1) and the cathode buffer K (pH 8.5 ±0.1). ROTI ® Blot 1 shows a much better transfer efficiency than a tris-glycine buffer and can be used for proteins and peptides of any length. Directions for use The blot stack is built up by soaking the upper blotting papers with cathode buffer and the lower blotting papers with anode buffer. With slightly longer transfer times, proteins >100 kDa can also be completely transferred with ROTI ® Blot 1. A detailed instruction manual is enclosed with the product. ROTI ® Blot K 10x conc., for electrophoresis
Product Detail
Glycine ≥99 %, Blotting Grade Glycin of very high purity, tested for absence of heavy metals, ammonia, and hydrolysable substances. Well soluble and particularly suitable for Western blotting.
Product Detail
Glycine ≥99 %, Blotting Grade Glycin of very high purity, tested for absence of heavy metals, ammonia, and hydrolysable substances. Well soluble and particularly suitable for Western blotting. Not a medical device / Not an IVD product
Product Detail
Amido black 10 B (C.I. 20470) p.a. Compatible with PVDF and NC membranes. Directions for use Staining solution: 0.5 % amido black in 50 % methanol (Art. No. 4627.1) / 10 % acetic acid (Art. No. 3738.1). Stain for 1-5 min at room temperature. Reduce background in 50 % methanol at room temperature.
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
