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<p>Adapted for use across diverse chemical handling environments, the Dna-Clickseq Library Prep Kit offers a versatile solution for next-generation sequencing (NGS). This kit employs click-chemistry in place of traditional enzymatic ligation methods, enabling the attachment of sequencing adapters to cDNA fragments during reverse transcription. Stored at -20°C, it caters to various input materials, including RNA and DNA, while boasting advantages such as no fragmentation steps, fewer processing steps, faster workflows, and an ultra-low chimera rate.</p> <ul> <li><strong>Category:</strong> NGS Kits</li> <li><strong>Storage temp.:</strong> -20 °C</li> </ul>
Product Detail
<p>Prepared for use in regulated laboratory conditions, the X-Clickseq Library Prep Kit is designed for next-generation sequencing (NGS) applications. This innovative solution employs click-chemistry rather than enzymes to attach sequencing adapters to cDNA during library preparation. By incorporating 3'-azido-nucleotides during reverse transcription, the cDNA strands are blocked at random points, facilitating subsequent click-ligation to alkyne-modified adapters. Following PCR amplification, the resulting libraries are ready for sequencing analysis. Notably, this method offers advantages such as reduced enzymatic ligation requirements, a streamlined workflow, and minimal chimera formation, making it an efficient choice for both RNA and DNA input samples. The kit includes essential components like x-Primer Mix, Click Mix, Click Accelerant, Click Catalyst, i7</p> <ul> <li><strong>Category:</strong> NGS Kits</li> <li><strong>Storage temp.:</strong> -20 °C</li> </ul>
Product Detail
<p>Adapted for use across diverse chemical handling environments, the Poly(A)-ClickSeq Library Prep Kit offers a streamlined approach for next-generation sequencing (NGS) applications focusing on polyadenylated RNA. This method, known as PAC-Seq, targets the 3' ends of eukaryotic mRNAs, providing cost-effective and efficient solutions for differential gene expression analysis and poly(A) site mapping. The kit employs 3'-azido-nucleotides (AzVTPs) during reverse transcription, followed by click-ligation to alkyne-functionalized adapters and PCR amplification to produce sequencing-ready libraries. Notably, this process eliminates the need for RNA purification, selection, or fragmentation, simplifying the workflow while maintaining high efficiency. Storing the kit at -20°C ensures its longev</p> <ul> <li><strong>Category:</strong> NGS Kits</li> <li><strong>Storage temp.:</strong> -20 °C</li> </ul>
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