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Analog of galactose, not split by ß-galactosidase. Inducer of the lac operon in bacteria. Used in conjunction with X-Gal(5-bromo-4-chloro-indolyl-ß-D-galactoside, cat. no. 15243) for detection of lac+ colonies. Dissolve in H20 to 200 mg/ml, sterilize by filtration and store in aliquots at -20 °C. For detection of transformants, use in final concentration of 0.1 mM. Analog of galactose, not split by ß-galactosidase. Inducer of the lac operon in bacteria. Used in conjunction with X-Gal(5-bromo-4-chloro-indolyl-ß-D-galactoside, cat. no. 15243) for detection of lac+ colonies. Dissolve in H20 to 200 mg/ml, sterilize by filtration and store in aliquots at -20 °C. For detection of transformants, use in final concentration of 0.1 mM.
Product Detail
X-Gal is a well known histochemical substrate used to detect the ß-galactosidase enzyme (1, 2, 3, 4, 9, 10). Identification of lacf-colonies (8). X-gal is used to distinguish recombinant plasmids from parental vectors in cloning experiments using vectors containing the lacZ or lacZ [DMvP1](-)peptide gene (2, 3, 5). Upon hydrolysis, X-Gal yields a localized, insoluble blue precipitate making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. X-Gal has been used for the detection of coliforms (E. coli) in municipal water supplies (6) and food products (7). X-Gal is often used in conjunction with IPTG (Isopropyl-ß-D-thiogalactopyranoside, cat. no. 26600) which binds and inhibits the lac repressor thus inducing ß-galactosidase activity.Stock solution: 20 mg/ml in DMF. Store in aliquots at -20 °C in the dark. For detection of transformants, use in final concentration of 40 µg/ml. X-Gal is a well known histochemical substrate used to detect the ß-galactosidase enzyme (1, 2, 3, 4, 9, 10). Identification of lacf-colonies (8). X-gal is used to distinguish recombinant plasmids from parental vectors in cloning experiments using vectors containing the lacZ or lacZ [DMvP1](-)peptide gene (2, 3, 5). Upon hydrolysis, X-Gal yields a localized, insoluble blue precipitate making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. X-Gal has been used for the detection of coliforms (E. coli) in municipal water supplies (6) and food products (7). X-Gal is often used in conjunction with IPTG (Isopropyl-ß-D-thiogalactopyranoside, cat. no. 26600) which binds and inhibits the lac repressor thus inducing ß-galactosidase activity.Stock solution: 20 mg/ml in DMF. Store in aliquots at -20 °C in the dark. For detection of transformants, use in final concentration of 40 µg/ml.
Product Detail
Analog of galactose, not split by ß-galactosidase. Inducer of the lac operon in bacteria. Used in conjunction with X-Gal(5-bromo-4-chloro-indolyl-ß-D-galactoside, cat. no. 15243) for detection of lac+ colonies. Dissolve in H20 to 200 mg/ml, sterilize by filtration and store in aliquots at -20 °C. For detection of transformants, use in final concentration of 0.1 mM. Analog of galactose, not split by ß-galactosidase. Inducer of the lac operon in bacteria. Used in conjunction with X-Gal(5-bromo-4-chloro-indolyl-ß-D-galactoside, cat. no. 15243) for detection of lac+ colonies. Dissolve in H20 to 200 mg/ml, sterilize by filtration and store in aliquots at -20 °C. For detection of transformants, use in final concentration of 0.1 mM.
Product Detail
X-Gal is a well known histochemical substrate used to detect the ß-galactosidase enzyme (1, 2, 3, 4, 9, 10). Identification of lacf-colonies (8). X-gal is used to distinguish recombinant plasmids from parental vectors in cloning experiments using vectors containing the lacZ or lacZ [DMvP1](-)peptide gene (2, 3, 5). Upon hydrolysis, X-Gal yields a localized, insoluble blue precipitate making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. X-Gal has been used for the detection of coliforms (E. coli) in municipal water supplies (6) and food products (7). X-Gal is often used in conjunction with IPTG (Isopropyl-ß-D-thiogalactopyranoside, cat. no. 26600) which binds and inhibits the lac repressor thus inducing ß-galactosidase activity.Stock solution: 20 mg/ml in DMF. Store in aliquots at -20 °C in the dark. For detection of transformants, use in final concentration of 40 µg/ml. X-Gal is a well known histochemical substrate used to detect the ß-galactosidase enzyme (1, 2, 3, 4, 9, 10). Identification of lacf-colonies (8). X-gal is used to distinguish recombinant plasmids from parental vectors in cloning experiments using vectors containing the lacZ or lacZ [DMvP1](-)peptide gene (2, 3, 5). Upon hydrolysis, X-Gal yields a localized, insoluble blue precipitate making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. X-Gal has been used for the detection of coliforms (E. coli) in municipal water supplies (6) and food products (7). X-Gal is often used in conjunction with IPTG (Isopropyl-ß-D-thiogalactopyranoside, cat. no. 26600) which binds and inhibits the lac repressor thus inducing ß-galactosidase activity.Stock solution: 20 mg/ml in DMF. Store in aliquots at -20 °C in the dark. For detection of transformants, use in final concentration of 40 µg/ml.
Product Detail
Substrate for horseradish peroxidase. More sensitive than ABTS and more stable and less toxic than diaminobenzidine. See also TMB Ready-To-Use ELISA Substrate (cat. no. 37068) and SERVAColor TMB Blot Solution (cat. no. 37071). Substrate for horseradish peroxidase. More sensitive than ABTS and more stable and less toxic than diaminobenzidine. See also TMB Ready-To-Use ELISA Substrate (cat. no. 37068) and SERVAColor TMB Blot Solution (cat. no. 37071).
Product Detail
Substrate for horseradish peroxidase. More sensitive than ABTS and more stable and less toxic than diaminobenzidine. See also TMB Ready-To-Use ELISA Substrate (cat. no. 37068) and SERVAColor TMB Blot Solution (cat. no. 37071). Substrate for horseradish peroxidase. More sensitive than ABTS and more stable and less toxic than diaminobenzidine. See also TMB Ready-To-Use ELISA Substrate (cat. no. 37068) and SERVAColor TMB Blot Solution (cat. no. 37071).
Product Detail
Analog of galactose, not split by ß-galactosidase. Inducer of the lac operon in bacteria. Used in conjunction with X-Gal(5-bromo-4-chloro-indolyl-ß-D-galactoside, cat. no. 15243) for detection of lac+ colonies. Dissolve in H20 to 200 mg/ml, sterilize by filtration and store in aliquots at -20 °C. For detection of transformants, use in final concentration of 0.1 mM. Analog of galactose, not split by ß-galactosidase. Inducer of the lac operon in bacteria. Used in conjunction with X-Gal(5-bromo-4-chloro-indolyl-ß-D-galactoside, cat. no. 15243) for detection of lac+ colonies. Dissolve in H20 to 200 mg/ml, sterilize by filtration and store in aliquots at -20 °C. For detection of transformants, use in final concentration of 0.1 mM.
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
