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Tested for use in tissue culture.At biological pH, HEPES is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 20 = 7.55). Thus, this Good's buffer is used for a wide range of applications in biological research: • In tissue culture to buffer cell culture media in air • As a component in cell culture media like DMEM, RPMI, Hanks’ solution • In biological solutions for the adjustment and maintenance of pH • For homogenization of tissue and in the preparation of cytosolic and nuclear cell extracts • As a buffer component in gel filtration and affinity chromatography Tested for use in tissue culture.At biological pH, HEPES is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 20 = 7.55). Thus, this Good's buffer is used for a wide range of applications in biological research: • In tissue culture to buffer cell culture media in air • As a component in cell culture media like DMEM, RPMI, Hanks’ solution • In biological solutions for the adjustment and maintenance of pH • For homogenization of tissue and in the preparation of cytosolic and nuclear cell extracts • As a buffer component in gel filtration and affinity chromatography
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pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies. pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies.
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MES is a widely used zwitterionic N-substituted aminosulfonic acid Good‘s buffer (1) with a pH range of 5.5 - 6.7 and a pKa of 6.15 at 20 °C.It is the ideal buffer substance for media and protein-based buffer formulations to maintain a stable environment in solution. Morpholinoethane sulfonic acid is used as a biological buffer in plant cell cultures, MES is also used in electrophoresis as an SDS PAGE running buffer for the resolution of small proteins and peptides.This is a high purity grade for biochemistry and molecular biology. MES is non-toxic to cells, highly water soluble and offers high solution clarity. Due to its minimal lipid solubility, MES is impermeable to membranes.Buffer solutions can be prepared with MES free acid by titrating the free acid with sodium hydroxide to the desired pH or by mixing equimolar volumes of MES free acid and sodium MES. When MES solutions are autoclaved, they turn yellow. Therefore, autoclaving is not recommended. To prepare nuclease-free buffers, treat the water first and then add the buffer after autoclaving. MES is a widely used zwitterionic N-substituted aminosulfonic acid Good‘s buffer (1) with a pH range of 5.5 - 6.7 and a pKa of 6.15 at 20 °C.It is the ideal buffer substance for media and protein-based buffer formulations to maintain a stable environment in solution. Morpholinoethane sulfonic acid is used as a biological buffer in plant cell cultures, MES is also used in electrophoresis as an SDS PAGE running buffer for the resolution of small proteins and peptides.This is a high purity grade for biochemistry and molecular biology. MES is non-toxic to cells, highly water soluble and offers high solution clarity. Due to its minimal lipid solubility, MES is impermeable to membranes.Buffer solutions can be prepared with MES free acid by titrating the free acid with sodium hydroxide to the desired pH or by mixing equimolar volumes of MES free acid and sodium MES. When MES solutions are autoclaved, they turn yellow. Therefore, autoclaving is not recommended. To prepare nuclease-free buffers, treat the water first and then add the buffer after autoclaving.
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Tris(hydroxymethyl)aminomethane (Tris) of ultrapure buffer quality for sensitive assays in molecular biology with very low UV absorption, DNase, RNase, Protease not detected.With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5.It is used as a component in buffer solutions in numerous applications: • Assays in molecular biology and cytology • Extraction of proteins or nucleic acids from cells • In situ hybridisation procedures • Sample and running buffers for SDS-PAGE • TAE- and TBE buffers for agarose gel electrophoresis • Transfer buffer for Western Blotting • Washing buffers for immunoassays Tris(hydroxymethyl)aminomethane (Tris) of ultrapure buffer quality for sensitive assays in molecular biology with very low UV absorption, DNase, RNase, Protease not detected.With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5.It is used as a component in buffer solutions in numerous applications: • Assays in molecular biology and cytology • Extraction of proteins or nucleic acids from cells • In situ hybridisation procedures • Sample and running buffers for SDS-PAGE • TAE- and TBE buffers for agarose gel electrophoresis • Transfer buffer for Western Blotting • Washing buffers for immunoassays
Product Detail
pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies. pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies.
Product Detail
pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies. pKa 20 = 7.2. Buffering substance for biochemistry, molecular and cell biology. Buffer component for isoelectric focussing and SDS PAGE. Substitute for HCO3-/CO2-buffer in cell culture for muscle studies.
Product Detail
Tris(hydroxymethyl)aminomethane (Tris) of ultrapure buffer quality with very low UV absorption, suitable for biological, enzymatic and pharmaceutical research.With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5.It is used as a component in buffer solutions in numerous applications: • Assays in molecular biology and cytology • Extraction of proteins or nucleic acids from cells • In situ hybridisation procedures • Sample and running buffers for SDS-PAGE • TAE- and TBE buffers for agarose gel electrophoresis • Transfer buffer for Western Blotting • Washing buffers for immunoassays Tris(hydroxymethyl)aminomethane (Tris) of ultrapure buffer quality with very low UV absorption, suitable for biological, enzymatic and pharmaceutical research.With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5.It is used as a component in buffer solutions in numerous applications: • Assays in molecular biology and cytology • Extraction of proteins or nucleic acids from cells • In situ hybridisation procedures • Sample and running buffers for SDS-PAGE • TAE- and TBE buffers for agarose gel electrophoresis • Transfer buffer for Western Blotting • Washing buffers for immunoassays
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USP. Tris(hydroxymethyl)aminomethane (Tris) buffer quality for all standard applications, suitable for preparative purposes and for chromatography.With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5. It is used as a component in buffer solutions in numerous applications: • Assays in molecular biology and cytology • Extraction of proteins or nucleic acids from cells • In situ hybridisation procedures • Sample and running buffers for SDS-PAGE • TAE- and TBE buffers for agarose gel electrophoresis • Transfer buffer for Western Blotting • Washing buffers for immunoassays USP. Tris(hydroxymethyl)aminomethane (Tris) buffer quality for all standard applications, suitable for preparative purposes and for chromatography.With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5. It is used as a component in buffer solutions in numerous applications: • Assays in molecular biology and cytology • Extraction of proteins or nucleic acids from cells • In situ hybridisation procedures • Sample and running buffers for SDS-PAGE • TAE- and TBE buffers for agarose gel electrophoresis • Transfer buffer for Western Blotting • Washing buffers for immunoassays
Product Detail
Tris(hydroxymethyl)aminomethane (Tris) of ultrapure buffer quality with very low UV absorption, suitable for biological, enzymatic and pharmaceutical research.With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5.It is used as a component in buffer solutions in numerous applications: • Assays in molecular biology and cytology • Extraction of proteins or nucleic acids from cells • In situ hybridisation procedures • Sample and running buffers for SDS-PAGE • TAE- and TBE buffers for agarose gel electrophoresis • Transfer buffer for Western Blotting • Washing buffers for immunoassays Tris(hydroxymethyl)aminomethane (Tris) of ultrapure buffer quality with very low UV absorption, suitable for biological, enzymatic and pharmaceutical research.With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5.It is used as a component in buffer solutions in numerous applications: • Assays in molecular biology and cytology • Extraction of proteins or nucleic acids from cells • In situ hybridisation procedures • Sample and running buffers for SDS-PAGE • TAE- and TBE buffers for agarose gel electrophoresis • Transfer buffer for Western Blotting • Washing buffers for immunoassays
Product Detail
Tris(hydroxymethyl)aminomethane hydrochloride (Tris HCl) of ultrapure buffer quality for biochemistry and enzymology with low UV absorption. Tris/Tris HCl is a commonly used buffering system for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology. Tris(hydroxymethyl)aminomethane hydrochloride (Tris HCl) of ultrapure buffer quality for biochemistry and enzymology with low UV absorption. Tris/Tris HCl is a commonly used buffering system for biopharmaceutical manufacturing, cell culture, diagnostics and molecular biology.
Product Detail
Buffering substance for biochemistry and enzymology. Buffering substance for biochemistry and enzymology.
Product Detail
Tris(hydroxymethyl)aminomethane (Tris) of ultrapure buffer quality with very low UV absorption, suitable for biological, enzymatic and pharmaceutical research.With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5.It is used as a component in buffer solutions in numerous applications: • Assays in molecular biology and cytology • Extraction of proteins or nucleic acids from cells • In situ hybridisation procedures • Sample and running buffers for SDS-PAGE • TAE- and TBE buffers for agarose gel electrophoresis • Transfer buffer for Western Blotting • Washing buffers for immunoassays Tris(hydroxymethyl)aminomethane (Tris) of ultrapure buffer quality with very low UV absorption, suitable for biological, enzymatic and pharmaceutical research.With a pKa of 8.1 Tris base is optimal for preparation of buffers in the physiological pH range of 7.3 to 7.5.It is used as a component in buffer solutions in numerous applications: • Assays in molecular biology and cytology • Extraction of proteins or nucleic acids from cells • In situ hybridisation procedures • Sample and running buffers for SDS-PAGE • TAE- and TBE buffers for agarose gel electrophoresis • Transfer buffer for Western Blotting • Washing buffers for immunoassays
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