Urea is a chaotropic agent used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. The reagent may be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. In denaturing isoelectric focusing and 2D-electrophoresisused urea is utilized to solubilize and denature proteins.Suitable for pharmaceutical research. Urea is a chaotropic agent used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. The reagent may be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. In denaturing isoelectric focusing and 2D-electrophoresisused urea is utilized to solubilize and denature proteins.Suitable for pharmaceutical research.