The Corning CellCube system provides a fast, simple, and compact method for the mass culture of attachment-dependent cells. It uses a tissue culture-treated growth surface for cell attachment, and continually perfuses the cells with fresh medium for increased cell productivity. The CellCube system provides an environment which more closely simulates in vivo conditions and reliably distributes nutrients and oxygen with low differential gradients across all cells within the modules. The CellCube modules provide a traditional tissue culture-treated surface or Corning CellBIND® surface for the growth of attachment dependent cells. The Corning CellCube system provides a fast, simple, and compact method for the mass culture of attachment-dependent cells. It uses a tissue culture-treated growth surface for cell attachment, and continually perfuses the cells with fresh medium for increased cell productivity. The CellCube system provides an environment which more closely simulates in vivo conditions and reliably distributes nutrients and oxygen with low differential gradients across all cells within the modules. The CellCube modules provide a traditional tissue culture-treated surface or Corning CellBIND® surface for the growth of attachment dependent cells.