Guanidine thiocyanate (GTC) is a chaotropic agent and strong denaturant. It solubilizes proteins by interfering with the hydrogen bonds and intramolecular interactions resulting in disintegration of cellular structures. GTC is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures RNase and can therefore be used for extraction of RNA. Guanidine thiocyanate (GTC) is a chaotropic agent and strong denaturant. It solubilizes proteins by interfering with the hydrogen bonds and intramolecular interactions resulting in disintegration of cellular structures. GTC is involved in separation of ribosomal ribonucleic acid (rRNA) from ribosomes and denatures RNase and can therefore be used for extraction of RNA.