Ready-to-use, gas-stabilized bisacrylamide ready-to-use solution. The ROTIPHORESE ® gel solution is very easy to use and enables reproducible and high-resolution gel electrophoresis. ROTIPHORESE ® gel solutions can be used for protein and nucleic acid separation in PAGE gels. The high-purity solutions can be combined with any buffer system. During sequencing, they facilitate the preparation of sequencing gels of consistent quality; our fluorescence-free gel solutions are recommended for the use of automatic sequencers. The ROTIPHORESE ® acrylamide and bisacrylamide solutions from Carl ROTH are produced only from acrylic acid-free substances and are strictly controlled in acrylamide content and pH. Oxidation products are removed from the solution by a selective absorption process. Their new formation and the polymerization of the solution are prevented by stabilizing the solution with inert gas. In order to ensure a high consistency of our ROTIPHORESE ® solutions, each batch is subjected to an electrophoretic test. Mechanism PAGE solutions are always a mixture of acrylamide and bisacrylamide. Acrylamide forms linear polymers, whereas bisacrylamide crosslinks these linear polymers forming pores of a special size. The pore size may be altered by varying the total gel concentration (% T) and the percentage of the crosslink (% C). With the ROTIPHORESE ® stock solutions the desired T/C rate can be produced. You need an acrylamide stock solution (Gel A or Gel A-40) and a bisacrylamide stock solution (Gel B). Acrylamide and Bisacrylamide Stock Solutions PAGE solutions are always a mixture of acrylamide and bisacrylamide. Acrylamide forms linear polymers, whereas bisacrylamide crosslinks these linear polymers forming pores of a special size. The pore size may be altered by varying the total gel concentration (% T) and the percentage of the crosslink (% C). With the ROTIPHORESE ® stock solutions the desired T/C rate can be produced. You need an acrylamide stock solution (Gel A or Gel A-40) and a bisacrylamide stock solution (Gel B). For detailed instructions regarding gel preparation with ROTIPHORESE ® Gel A, A-40 and B please see user manual and technical information brochure “PAGE Instructions”. ROTIPHORESE ® Gel B ROTIPHORESE ® 2 %, gas-stabilized Directions for use Ready-to-use, stabilised 2 % bisacrylamide stock solution. In combination with ROTIPHORESE ® Gel A (30 % acrylamide stock solution, Art. No. 3037) or ROTIPHORESE ® Gel A-40 (40 % acrylamide stock solution, Art. No. 7748) gels with up to 25 % total acrylamide concentration can be produced. For detailed instructions regarding gel preparation with ROTIPHORESE ® Gel A, A-40 and B please see our Technical Information Brochure “PAGE Instructions”.