Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale. Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep) High affinity to the respective protein tag Robust & highly specific detection of the fusion protein Unconjugated Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence anti-c-myc-tag mouse monoclonal, 9E10 lyophilized, monoclonal, affinity purified The monoclonal 9E10 antibody recognizes human c-myc, a 62 kDa transcription factor. A c-myc tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein. Application Immunoprecipitation (IP): assay dependent Western Blot (WB): 1:50,000-1:100,000 (0.005-0.0025 μg/ml) Western blot analysis of E. coli lysate containing c-myc-tagged protein with anti-c-myc-tag antibody. Western blot analysis was performed on 80 ng, 40 ng, 10 ng or 5 ng of E. coli lysate containing c-myc-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5% dry milk in PBST for 1 h at RT. The primary antibody anti-c-myc-tag mouse monoclonal, 9E10 (Cat. No. 910MYCL) was diluted in blocking buffer (antibody concentration 0.005 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate. Not a medical device / Not an IVD product