Produced for stability across temperature-sensitive applications, the Hplc Column Nucleodur® C 4 Ec, 250 mm, 4 mm features a medium density butyl modification based on high purity NUCLEODUR® silica gel. This separation column, with a particle size of 5 µm and fully porous particles (FPP), is ideal for the separation of biomolecules such as proteins and peptides. With a specific surface area of 100 m²/g according to BET, it offers excellent interaction and retention mechanisms via hydrophobic van der Waals interactions and some residual silanol interactions. The column's pressure stability of 450 bar ensures reliable performance under various conditions, while its recommended eluent is acetonitrile-water. The Hplc Column Nucleodur® C 4 Ec, 250

• Category: Separation columns for HPLC
• Column type: HPLC column
• Temperature stability: 60 ° C
• Particle type: Fully porous particles (FPP)
• Pore size: 300 Å
• Phase: C 4 ec
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• Endcapped: yes
• Separation principle: Reversed phase (RP)
• Column volume: 3.15 ml
• Specific surface according to BET: 100 m²/g
• L x Ø internal (column): 250 x 4 mm
• Surface chemistry: Medium density monomeric butyl (C 4 ) wide-pore modification
• Application Database (Macherey-Nagel): HPLC
• Particle size: 5 µm
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions
• pH stability: 1,0-9,0
• Pore volume: 0.9 ml/g
• Similar phases: Jupiter ® C 4 ACE ® C 4
• Pressure stability: 450 bar
• USP listing: L26
• Eluent in column: Acetonitrile - Water
• Column shape: Analytical separation column