Produced to ensure dependable processing results, the Hplc Column Nucleodur ® Hilic, 3 μm, 150 mm, 4.6 mm features a hydrophilic interaction chromatography (HILC) separation principle and ammonium – sulfonic acid (zwitterionic) surface chemistry. This analytical separation column has a specific surface area of 340 m²/g as per BET, a pore size of 110 Å, and a pore volume of 0.9 ml/g. It operates at temperatures up to 60°C and pressure stabilities of 600 bar. With a recommended application range including highly polar analytes, water-soluble vitamins, and organic polar acids and bases, this column is ideal for separating polar compounds that may be challenging using traditional reversed-phase HPLC

• Category: Separation columns for HPLC
• Particle type: Fully porous particles (FPP)
• Pore size: 110 Å
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Pore volume: 0.9 ml/g
• Specific surface according to BET: 340 m²/g
• Column type: HPLC column
• Pressure stability: 600 bar
• USP listing: Not available or proprietary
• Endcapped: no
• L x Ø internal (column): 150 x 4.6 mm
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Phase: HILIC
• Eluent in column: Acetonitrile - Water
• Temperature stability: 60 ° C
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Particle size: 3 µm
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Column volume: 2.5 ml
• Column shape: Analytical separation column
• pH stability: 2,0-8,5