Manufactured to meet research and production requirements, this HPLC column Nucleodur® Hilic features a particle size of 3 μm, a length of 125 mm, and an inner diameter of 4.6 mm. It is designed for use in separating highly polar analytes such as amino acids, peptides, water-soluble vitamins, and organic polar acids and bases. This column employs the hydrophilic interaction chromatography (HILIC) technique, which is particularly useful for separating polar compounds that may be challenging using traditional reversed-phase HPLC methods. Its surface chemistry consists of an ammonium – sulfonic acid (zwitterionic) modification, ensuring optimal performance for a wide range of applications. With a specific surface area of 340 m²/g and a pH stability ranging from 2.0 to

• Category: Separation columns for HPLC
• Column volume: 2.06 ml
• Eluent in column: Acetonitrile - Water
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• USP listing: Not available or proprietary
• pH stability: 2,0-8,5
• Endcapped: no
• Column shape: Analytical separation column
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Phase: HILIC
• Pore volume: 0.9 ml/g
• Particle size: 3 µm
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Temperature stability: 60 ° C
• Column type: HPLC column
• Pressure stability: 600 bar
• Pore size: 110 Å
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Specific surface according to BET: 340 m²/g
• L x Ø internal (column): 125 x 4.6 mm
• Particle type: Fully porous particles (FPP)