Intended for structured formulation workflows, the Hplc Column Nucleodur® C 4 Ec, 150 mm, 2 mm offers an ideal solution for separating biomolecules such as proteins and peptides. This reversed-phase (RP) column features a medium density monomeric butyl (C 4 ) wide-pore modification, ensuring lower retention for hydrophobic compounds compared to other common modifications like C 18 or C 8. With a specific surface area of 100 m²/g, this analytical separation column boasts a pressure stability of 450 bar and temperature stability up to 60°C. The column's particle size of 5 µm, combined with its fully porous particles (FPP) design, ensures optimal performance under various conditions. Delivered with an instruction leaflet and certificate of analysis,

• Category: Separation columns for HPLC
• Particle type: Fully porous particles (FPP)
• Pore size: 300 Å
• Specific surface according to BET: 100 m²/g
• Interactions / retention mechanism: hydrophobic (van der Waals interactions), some residual silanol interactions
• Column shape: Analytical separation column
• Pressure stability: 450 bar
• Column type: HPLC column
• Particle size: 5 µm
• USP listing: L26
• Eluent in column: Acetonitrile - Water
• pH stability: 1,0-9,0
• Surface chemistry: Medium density monomeric butyl (C 4 ) wide-pore modification
• Recommended application(s): Biomolecular macromolecules, like proteins and peptides
• Column volume: 0.45 ml
• Phase: C 4 ec
• Similar phases: Jupiter ® C 4 ACE ® C 4
• Endcapped: yes
• Separation principle: Reversed phase (RP)
• L x Ø internal (column): 150 x 2 mm
• Pore volume: 0.9 ml/g
• Temperature stability: 60 ° C