Designed with efficiency in synthetic processes in mind, this HPLC column Nucleodur ® Hilic features a particle size of 3 μm and a length of 125 mm, providing a column volume of 0.87 ml. The column's surface chemistry is ammonium – sulfonic acid (zwitterionic) modified, making it suitable for separating highly polar analytes such as amino acids, peptides, and water-soluble vitamins. With a pH stability range of 2.0-8.5 and a temperature stability of 60 °C, this column is versatile and durable. It operates under Hydrophilic Interaction Chromatography (HILIC) principles, utilizing ionic and hydrophilic interactions for retention mechanisms. This column is ideal for those seeking efficient separation of polar compounds in their synthetic processes.

• Category: Separation columns for HPLC
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Column volume: 0.87 ml
• Phase: HILIC
• Specific surface according to BET: 340 m²/g
• Pore volume: 0.9 ml/g
• Pressure stability: 600 bar
• Column shape: Analytical separation column
• L x Ø internal (column): 125 x 3 mm
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Particle size: 3 µm
• USP listing: Not available or proprietary
• Pore size: 110 Å
• Application Database (Macherey-Nagel): HPLC SPE
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• Endcapped: no
• pH stability: 2,0-8,5
• Temperature stability: 60 ° C
• Eluent in column: Acetonitrile - Water
• Particle type: Fully porous particles (FPP)
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• Column type: HPLC column