Developed for demanding processing applications, the HPLC Column Nucleodur® Hilic, 5 μm, 50 mm, 4.6 mm offers exceptional performance for separating highly polar analytes such as amino acids, peptides, water-soluble vitamins, and organic polar acids and bases. This analytical separation column features a hydrophilic interaction chromatography (HILIC) phase based on high purity NUCLEODUR® silica gel, providing an alternative strategy for modern analytics, particularly when reversed phase HPLC encounters limitations with polar compounds. With a specific surface area of 340 m²/g, this fully porous particle (FPP) column boasts a pore size of 110 Å and a pore volume of 0.9 ml/g, ensuring optimal separation efficiency. The column's pressure stability

• Category: Separation columns for HPLC
• Pressure stability: 600 bar
• Eluent in column: Acetonitrile - Water
• Specific surface according to BET: 340 m²/g
• Particle size: 5 µm
• Particle type: Fully porous particles (FPP)
• Endcapped: no
• Temperature stability: 60 ° C
• Recommended application(s): Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases Peptides Polar natural products Water-soluble vitamins
• pH stability: 2,0-8,5
• USP listing: Not available or proprietary
• Pore size: 110 Å
• Separation principle: Hydrophilic Interaction Chromatography (HILIC)
• L x Ø internal (column): 50 x 4.6 mm
• Interactions / retention mechanism: ionic / hydrophilic and electrostatic
• Column type: HPLC column
• Pore volume: 0.9 ml/g
• Phase: HILIC
• Surface chemistry: Ammonium – sulfonic acid (zwitterionic) modification
• Column volume: 0.83 ml
• Column shape: Analytical separation column