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For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system. Molar extinction coefficient: 4,4x10 4 L x mol -1 x cm -1 (acc. to 0,667 L x mol -1 x cm -1 for a 0,1 % solution). Bovine Serum Albumin (BSA) Fraction V, pH 5.2, US-Origin ≥96 % The Albumin, pH 5.2, US-Origin is very well suited for all applications where Albumin is used as a stabilising reagent, for blocking or as a carrier, e.g. for stabilisation of enzymes and antibodies, or as hapten carrier in immunoassays. The pH value of albumin pH 5.2 US-Origin is close to its pI and the protein is only weakly charged. It is, therefore, particularly recommended for use in ELISA and Western blotting, since it may help in further reducing background signals.
Product Detail
Protein-free blocking solution for all blot and hybridisation systems. Diluted also perfectly suitable as base solution for immunochemical detections. Suitable for: Western- and Far Western Analysis Southern- and Northern-Blots Dot-Blots ELISA- and Sandwich-Assays Precoating of ELISA-plates (incl. drying) in situ hybridisation Microarray hybridisation In order to obtain high signal intensity, nonspecific antibody binding sites must be blocked. Whilst detergents are not effective as blocking agents, the addition of proteins such as BSA may mask specific binding sites. ROTI ® Block is an innovative, polymer-based blocking reagent which solves such problems. With ROTI ® Block, the specific binding sites remain accessible while nonspecific reactions are suppressed, thus leading to an increase in signal intensity. ROTI ® Block 10x conc., ready-to-use ROTI ® Block is a protein-free blocking solution that can be used diluted as a base solution for all steps of Western detections and ELISAs. ROTI ® Block replaces PBS/TBS buffers and other buffer systems including the blocking reagents used. If required, ROTI ® Block can be enriched with further blocking reagents, detergents etc. to further optimise the detection system. Figure: Western blotting of decreasing amounts (50 ng - 17 pg) of a bacterial extract including a 40 kDa protein. Probed by Anti His Serum (1:1.000) and Carl ROTH Anti Rabbit-HRP, blocking by means of ROTI ® Block. Detection via chemoluminescence. Directions for use Included you will find a detailed instruction manual with protocols, e.g. for stripping ROTI ® PVDF-membranes from bound probes without destabilising the blocking, which enables fast rescreening of the filter. ROTI ® Block also enables the blot to be Ponceau stained even after blocking. For detection systems using antibodies specifically directed against protein-phosphorylations we recommend to test and optimise the blocking efficiency prior to assaying your samples.
Product Detail
For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system. Molar extinction coefficient: 4,4x10 4 L x mol -1 x cm -1 (acc. to 0,667 L x mol -1 x cm -1 for a 0,1 % solution). Bovine Serum Albumin (BSA) Fraction V, pH 5.2, US-Origin ≥96 % The Albumin, pH 5.2, US-Origin is very well suited for all applications where Albumin is used as a stabilising reagent, for blocking or as a carrier, e.g. for stabilisation of enzymes and antibodies, or as hapten carrier in immunoassays. The pH value of albumin pH 5.2 US-Origin is close to its pI and the protein is only weakly charged. It is, therefore, particularly recommended for use in ELISA and Western blotting, since it may help in further reducing background signals.
Product Detail
Powdered milk Blotting Grade, powdered, low in fat Lowfat powdered milk, especially suited for use as blocking reagent in immunological assays like Western-Blot analyses or ELISA. Fine powdered for easy application. Please note Milk contains biotin. Not suitable for biotin/streptavidine mediated detection systems. The added anti-caking agents (phosphates) guarantee easy weighing. Calcium, magnesium and iron phosphates can precipitate in higher concentrated solutions of milk powder.
Product Detail
For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system. Molar extinction coefficient: 4,4x10 4 L x mol -1 x cm -1 (acc. to 0,667 L x mol -1 x cm -1 for a 0,1 % solution). Bovine Serum Albumin (BSA) Fraction V, NZ-Origin CELLPURE ® ≥98 %, fatty acid-free, IgG-free, very low endotoxin Highly pure albumin, prepared from blood of cattle certified for origin from Australia or New Zealand. Albumin, fatty acid-free, NZ-Origin, may well be used for blocking of all assays regarding membrane proteins, or other approaches aiming for fatty acid-associated proteins. It is also well suited for stabilisation of antibodies, enzymes or fatty acids. Albumin, fatty acid-free, NZ-Origin, shows exceedingly low content of endotoxins and IgGs, making it a useful reagent for cell biology also. Tested for the presence of fatty acids, IgGs, and endotoxins.
Product Detail
For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system. Molar extinction coefficient: 4,4x10 4 L x mol -1 x cm -1 (acc. to 0,667 L x mol -1 x cm -1 for a 0,1 % solution). Bovine Serum Albumin (BSA) Fraction V, NZ-Origin CELLPURE ® ≥98 %, protease-free, fatty acid-free, IgG-free, very low endotoxin, nuclease-free Tested for the presence of proteases, fatty acids, IgGs, endotoxins, and DNases and RNases. Not a medical device / Not an IVD product
Product Detail
Powdered milk Blotting Grade, powdered, low in fat Lowfat powdered milk, especially suited for use as blocking reagent in immunological assays like Western-Blot analyses or ELISA. Fine powdered for easy application. Please note Milk contains biotin. Not suitable for biotin/streptavidine mediated detection systems. The added anti-caking agents (phosphates) guarantee easy weighing. Calcium, magnesium and iron phosphates can precipitate in higher concentrated solutions of milk powder.
Product Detail
For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system. Molar extinction coefficient: 4,4x10 4 L x mol -1 x cm -1 (acc. to 0,667 L x mol -1 x cm -1 for a 0,1 % solution). Bovine Serum Albumin (BSA) Fraction V, US-Origin ≥98 %, protease-free High purity albumin with origin from the USA with very low protease content. Excellent in sensitive immuno assays, as a stabilising reagent for proteins, enzymes and antibodies and also suitable as a blocking reagent in hybridisation techniques. Tested for the absence of detectable protease activity.
Product Detail
For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system. Molar extinction coefficient: 4,4x10 4 L x mol -1 x cm -1 (acc. to 0,667 L x mol -1 x cm -1 for a 0,1 % solution). Bovine Serum Albumin (BSA) Fraction V, NZ-Origin CELLPURE ® ≥98 %, fatty acid-free, IgG-free, very low endotoxin Highly pure albumin, prepared from blood of cattle certified for origin from Australia or New Zealand. Albumin, fatty acid-free, NZ-Origin, may well be used for blocking of all assays regarding membrane proteins, or other approaches aiming for fatty acid-associated proteins. It is also well suited for stabilisation of antibodies, enzymes or fatty acids. Albumin, fatty acid-free, NZ-Origin, shows exceedingly low content of endotoxins and IgGs, making it a useful reagent for cell biology also. Tested for the presence of fatty acids, IgGs, and endotoxins.
Product Detail
Protein-free blocking solution for all blot and hybridisation systems. Diluted also perfectly suitable as base solution for immunochemical detections. Suitable for: Western- and Far Western Analysis Southern- and Northern-Blots Dot-Blots ELISA- and Sandwich-Assays Precoating of ELISA-plates (incl. drying) in situ hybridisation Microarray hybridisation In order to obtain high signal intensity, nonspecific antibody binding sites must be blocked. Whilst detergents are not effective as blocking agents, the addition of proteins such as BSA may mask specific binding sites. ROTI ® Block is an innovative, polymer-based blocking reagent which solves such problems. With ROTI ® Block, the specific binding sites remain accessible while nonspecific reactions are suppressed, thus leading to an increase in signal intensity. ROTI ® Block 10x conc., ready-to-use ROTI ® Block is a protein-free blocking solution that can be used diluted as a base solution for all steps of Western detections and ELISAs. ROTI ® Block replaces PBS/TBS buffers and other buffer systems including the blocking reagents used. If required, ROTI ® Block can be enriched with further blocking reagents, detergents etc. to further optimise the detection system. Figure: Western blotting of decreasing amounts (50 ng - 17 pg) of a bacterial extract including a 40 kDa protein. Probed by Anti His Serum (1:1.000) and Carl ROTH Anti Rabbit-HRP, blocking by means of ROTI ® Block. Detection via chemoluminescence. Directions for use Included you will find a detailed instruction manual with protocols, e.g. for stripping ROTI ® PVDF-membranes from bound probes without destabilising the blocking, which enables fast rescreening of the filter. ROTI ® Block also enables the blot to be Ponceau stained even after blocking. For detection systems using antibodies specifically directed against protein-phosphorylations we recommend to test and optimise the blocking efficiency prior to assaying your samples.
Product Detail
For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system. Molar extinction coefficient: 4,4x10 4 L x mol -1 x cm -1 (acc. to 0,667 L x mol -1 x cm -1 for a 0,1 % solution). Bovine Serum Albumin (BSA) Fraction V, NZ-Origin CELLPURE ® ≥98 %, protease-free, fatty acid-free, IgG-free, very low endotoxin, nuclease-free Tested for the presence of proteases, fatty acids, IgGs, endotoxins, and DNases and RNases. Not a medical device / Not an IVD product
Product Detail
For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system. Molar extinction coefficient: 4,4x10 4 L x mol -1 x cm -1 (acc. to 0,667 L x mol -1 x cm -1 for a 0,1 % solution). Bovine Serum Albumin (BSA) Fraction V, US-Origin ≥98 %, protease-free High purity albumin with origin from the USA with very low protease content. Excellent in sensitive immuno assays, as a stabilising reagent for proteins, enzymes and antibodies and also suitable as a blocking reagent in hybridisation techniques. Tested for the absence of detectable protease activity.
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
