Blocking Reagents Southern Blotting

HP33.1, Carl Roth

Denhardt's solution, lyophilised powder 50x, BioScience Grade, for molecular biology Denhardt’s lyophilised powder contains a mixture of different blocking agents that is used for nuclear acid hybridisation (Northern and Southern Blot) as well as for stripping of hybridised membranes. By prehybridisation the filter membranes with Denhardt’s solution unspecific binding of DNA to the membrane is avoided. Directions for use For hybridisation use 10x Denhardt’s solution with 6x SSC (ROTI ® Stock 20x SSC, Art. No. 1054.1) supplemented with 100 µg/ml denatured salmon sperm DNA and 1 % SDS (ROTI ® Stock 20 % SDS, Art. No. 1057.1).