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Sterile ROTI ® Cell HEPES solution CELLPURE ® 1 M, sterile HEPES belongs to the “goods buffers” and its properties make it optimally suited as an additional buffer system in cell culture. Depending on the concentration, it stabilizes the pH value in the cell culture media in a range between 6 and 8.5. Therefore, the use of the ROTI ® Cell HEPES solution is particularly recommended when the pH value urgently needs to be kept constantly high. pH fluctuations mainly occur when working outside the CO 2 incubator for longer periods.
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Sterile ROTI ® Cell MEM-AA solution CELLPURE ® 50x conz., sterile, w/o glutamine ROTI ® Cell MEM-AA solution consists of a pre-formulated mixture of 12 essential amino acids in water perfectly suited for culturing of most eukaryotic cells. Since cells are not able to synthesize essential amino acids by themselves, those have to be supplemented externallyd. A 1:50 dilution of ROTI Cell MEM-AA solution in cell culture medium will give a final concentration for each amino acid corresponding to the amount in Eagle's Minimum Essential Medium.
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Sterile ROTI ® Cell Water CELLPURE ® ready-to-use, sterile Not a medical device / Not an IVD product
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Sterile ROTI ® Cell L-Alanyl-L-Glutamine solution CELLPURE ® 200 mM, sterile, stable L-glutamine is an essential amino acid that is an important source of energy in cell culture media and is indispensable for cell growth. Since normal L-glutamine is temperature sensitive and spontaneously decomposes to toxic ammonia in the course of cell cultivation, the stable ROTI ® Cell Glutamine Solution is an optimal alternative. Stable glutamine is present as the dipeptide L-alanyl-L-glutamine. Due to its reinforced structure, this form of glutamine is not temperature sensitive, unlike normal L-glutamine. Cells are able to break down this dipeptide bond and release L-glutamine as needed. Thus, the low ammonia concentration improves cell viability and growth. In addition, the concentration of L-glutamine is kept constant. ROTI ® Cell Glutamine Solution can be stored at room temperature for a long period of time.
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Sterile ROTI ® Cell Water CELLPURE ® ready-to-use, sterile Not a medical device / Not an IVD product
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Heparin sodium salt ≥180 I.U./mg, Ph. Eur., from pork intestine mucosa
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Sterile ROTI ® Cell Glucose Solution CELLPURE ® 250 g/L, sterile D-(+)-Glucose (dextrose) is a widely used natural sugar that serves as a supplement for cell culture as well as for numerous other cellular and molecular applications. Not a medical device / Not an IVD product
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Heparin sodium salt ≥180 I.U./mg, Ph. Eur., from pork intestine mucosa
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Sterile ROTI ® Cell Pyruvate solution CELLPURE ® 100 mM, sterile Sodium Pyruvate is a key intermediate in the ATP production within the cell and is sometimes added to cell culture media as an additional source of energy. Additional pyruvate may help cells deficient in one of the enzymes involved in glucose metabolism, and may kick-start cells in the citric acid cycle, thus supporting growth of low density cells. Concentration in standard media is 1 mM; special media like Leibovitz ’ s medium are supplemented with up to 5 mM pyruvate.
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Heparin sodium salt ≥180 I.U./mg, Ph. Eur., from pork intestine mucosa
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Sterile ROTI ® Cell Insulin solution CELLPURE ® 5 mg/ml, sterile, human recombinant Recombinant human insulin is commonly used as a chemical supplement for the cultivation of a variety of mammalian cell lines, particularly CHO cells. It regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids, and also inhibits the breakdown of glycogen, proteins, and fats. For human insulin: 1 U ≈ 0.0347 mg (biological activity, IU).For a 5 mg/mL insulin solution, this corresponds to an activity of approximately 144 U/mL. Expressed in yeast (Saccharomyces cerevisiae). Not a medical device / Not an IVD product
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Sterile ROTI ® Cell MEM-NEAA solution CELLPURE ® 100x conc., 10 mM (each), sterile ROTI ® Cell MEM-NEAA solution consists of a mixture of 7 non-essential amino acids in water. Enrichment of ROTI ® Cell Eagle ’ s MEM Medium (with Earle ’ s or Hanks ’ salts) with non-essential amino acids enables culturing of fastidious cells and cell lines, hereby broadening the utility of this medium. Dilution by 1:100 of ROTI ® Cell MEM-NEAA solution yields a final concentration 0.1 mM of each component.
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
