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Polysucrose 400 BioScience Grade, for molecular biology Nonionic, synthetic copolymer from sucrose and epichlorohydrin. Ficoll ® derivative. Solution of polysucrose 400 is used for separation of blood cells (mono nuclear cells, granulocytes, erythrocytes) via density gradient centrifugation, and for isolation of mononuclear cells from whole blood. Also well applicable for isolation of islets of Langerhans from pancreatic issue.
Product Detail
Ficoll ® 400 for molecular biology and biochemistry Ficoll ® 400 is a hydrophilic copolymer of saccharose and epichlorohydrin, that readily solubilises in water and aqueous solutions. It may be solubilised at concentrations of up to 50 %, and is mainly used in the following applications: A) In hybridisation buffer acc. to Denhardt, Ficoll ® 400 reduces non specific binding of labelled DNA to the filter membrane. B) In gel loading buffer it supports DNA application to agarose gels by increasing the density of the sample. C) In continuous or discontinuous density gradients, Ficoll ® 400 is used as matrix for fractioning of cells (e.g. lymphocytes) or subcellular particles, also taking advantage of its ability to stabilise membrane coupled components and the functional as well as the morphological integrity of cells. Autoclavable at neutral pH.
Product Detail
Sterile Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from mammalian blood by density gradient centrifugation. Density 1, 077 g/ml. Best alternative to Ficoll ® . Optimised for isolation of lymphocytes from non-coagulated mammaliam whole blood Produces distinct, compact layers Results in high recovery rates of viable cells Maintains the representative ratio of B- to T lymphocytes May be directly applied to all protocols using Ficoll ® ROTI ® Sep 1077 animal excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ration of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range. Suitable for application with non-coagulated peripheral whole blood from all mammals. In all respective protocols originally using Ficoll ® this reagent may be replaced by ROTI ® Sep 1077 animal without any adjustment. ROTI ® Sep 1077 animal CELLPURE ® sterile, ready-to-use, for density gradient centrifugation Not a medical device / Not an IVD product
Product Detail
Sterile Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from murine blood by density gradient centrifugation. Density 1, 086 g/ml. Best alternative to Ficoll ® . Optimised for isolation of lymphocytes from non-coagulated mouse whole blood Produces distinct, compact layers Results in high recovery rates of viable cells Maintains the representative ratio of B- to T lymphocytes May be directly applied to all protocols using Ficoll ® ROTI ® Sep 1086 mouse excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ration of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range. Suitable for application with non-coagulated peripheral whole blood from mouse. In all respective protocols originally using Ficoll ® this reagent may be replaced by ROTI ® Sep 1086 mouse without any adjustment. ROTI ® Sep 1086 mouse CELLPURE ® sterile, ready-to-use, for density gradient centrifugation Not a medical device / Not an IVD product
Product Detail
Polysucrose 400 BioScience Grade, for molecular biology Nonionic, synthetic copolymer from sucrose and epichlorohydrin. Ficoll ® derivative. Solution of polysucrose 400 is used for separation of blood cells (mono nuclear cells, granulocytes, erythrocytes) via density gradient centrifugation, and for isolation of mononuclear cells from whole blood. Also well applicable for isolation of islets of Langerhans from pancreatic issue.
Product Detail
Sterile Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from mammalian blood by density gradient centrifugation. Density 1, 077 g/ml. Best alternative to Ficoll ® . Optimised for isolation of lymphocytes from non-coagulated mammaliam whole blood Produces distinct, compact layers Results in high recovery rates of viable cells Maintains the representative ratio of B- to T lymphocytes May be directly applied to all protocols using Ficoll ® ROTI ® Sep 1077 animal excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ration of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range. Suitable for application with non-coagulated peripheral whole blood from all mammals. In all respective protocols originally using Ficoll ® this reagent may be replaced by ROTI ® Sep 1077 animal without any adjustment. ROTI ® Sep 1077 animal CELLPURE ® sterile, ready-to-use, for density gradient centrifugation Not a medical device / Not an IVD product
Product Detail
Sterile Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation. Density 1.077 g/ml. Best alternative to Ficoll ® . Optimised for isolation of lymphocytes from non-coagulated human whole blood Produces distinct, compact layers Results in high recovery rates of viable cells Maintains the representative ratio of B- to T lymphocytes May be directly applied to all protocols using Ficoll ® ROTI ® Sep 1077 human excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ratio of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range. Suitable for application with human non-coagulated peripheral blood. In all respective protocols originally using Ficoll ® this reagent may be replaced by ROTI ® Sep 1077 human without any adjustment. ROTI ® Sep 1077 human/tube CELLPURE ® sterile, ready-to-use, for density gradient centrifugation Prefilled in 50 ml centrifugation tubes, 15 ml each. With permeable membrane for easy pipetting of diluted blood. Not a medical device / Not an IVD product
Product Detail
Sterile Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation. Density 1.077 g/ml. Best alternative to Ficoll ® . Optimised for isolation of lymphocytes from non-coagulated human whole blood Produces distinct, compact layers Results in high recovery rates of viable cells Maintains the representative ratio of B- to T lymphocytes May be directly applied to all protocols using Ficoll ® ROTI ® Sep 1077 human excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ratio of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range. Suitable for application with human non-coagulated peripheral blood. In all respective protocols originally using Ficoll ® this reagent may be replaced by ROTI ® Sep 1077 human without any adjustment. ROTI ® Sep 1077 human CELLPURE ® sterile, ready-to-use, for density gradient centrifugation The figure depicts two specimens prepared with heparinic blood obtained from venous puncture. Density gradient centrifugation of 6 ml diluted whole blood (dilution in PBS 1:1.5), with 3 ml of ROTI ® Sep 1077 human, 10 mins., 1000 g, no break. Not a medical device / Not an IVD product
Product Detail
Ficoll ® 400 for molecular biology and biochemistry Ficoll ® 400 is a hydrophilic copolymer of saccharose and epichlorohydrin, that readily solubilises in water and aqueous solutions. It may be solubilised at concentrations of up to 50 %, and is mainly used in the following applications: A) In hybridisation buffer acc. to Denhardt, Ficoll ® 400 reduces non specific binding of labelled DNA to the filter membrane. B) In gel loading buffer it supports DNA application to agarose gels by increasing the density of the sample. C) In continuous or discontinuous density gradients, Ficoll ® 400 is used as matrix for fractioning of cells (e.g. lymphocytes) or subcellular particles, also taking advantage of its ability to stabilise membrane coupled components and the functional as well as the morphological integrity of cells. Autoclavable at neutral pH.
Product Detail
Sterile Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation. Density 1.077 g/ml. Best alternative to Ficoll ® . Optimised for isolation of lymphocytes from non-coagulated human whole blood Produces distinct, compact layers Results in high recovery rates of viable cells Maintains the representative ratio of B- to T lymphocytes May be directly applied to all protocols using Ficoll ® ROTI ® Sep 1077 human excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ratio of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range. Suitable for application with human non-coagulated peripheral blood. In all respective protocols originally using Ficoll ® this reagent may be replaced by ROTI ® Sep 1077 human without any adjustment. ROTI ® Sep 1077 human CELLPURE ® sterile, ready-to-use, for density gradient centrifugation The figure depicts two specimens prepared with heparinic blood obtained from venous puncture. Density gradient centrifugation of 6 ml diluted whole blood (dilution in PBS 1:1.5), with 3 ml of ROTI ® Sep 1077 human, 10 mins., 1000 g, no break. Not a medical device / Not an IVD product
Product Detail
Sterile Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from murine blood by density gradient centrifugation. Density 1, 086 g/ml. Best alternative to Ficoll ® . Optimised for isolation of lymphocytes from non-coagulated mouse whole blood Produces distinct, compact layers Results in high recovery rates of viable cells Maintains the representative ratio of B- to T lymphocytes May be directly applied to all protocols using Ficoll ® ROTI ® Sep 1086 mouse excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ration of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range. Suitable for application with non-coagulated peripheral whole blood from mouse. In all respective protocols originally using Ficoll ® this reagent may be replaced by ROTI ® Sep 1086 mouse without any adjustment. ROTI ® Sep 1086 mouse CELLPURE ® sterile, ready-to-use, for density gradient centrifugation Not a medical device / Not an IVD product
Product Detail
Ficoll ® 400 for molecular biology and biochemistry Ficoll ® 400 is a hydrophilic copolymer of saccharose and epichlorohydrin, that readily solubilises in water and aqueous solutions. It may be solubilised at concentrations of up to 50 %, and is mainly used in the following applications: A) In hybridisation buffer acc. to Denhardt, Ficoll ® 400 reduces non specific binding of labelled DNA to the filter membrane. B) In gel loading buffer it supports DNA application to agarose gels by increasing the density of the sample. C) In continuous or discontinuous density gradients, Ficoll ® 400 is used as matrix for fractioning of cells (e.g. lymphocytes) or subcellular particles, also taking advantage of its ability to stabilise membrane coupled components and the functional as well as the morphological integrity of cells. Autoclavable at neutral pH.
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