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ROTI ® Fect CELLPURE ® ready-to-use, for transfection ROTI ® Fect is a liposome-based transfection reagent consisting of a mixture of polycationic and neutral lipids. Compared to monocationic lipid formulations, ROTI ® Fect has a higher efficiency with lower levels being required. Its excellent reproducibility in combination with its high level of efficiency reduced optimisation work to a minimum. Because of its low toxicity, a wide range of cell lines can be transfected efficiently. An efficient triple-cotransfection using ROTI ® Fect is described in Ufartes, Schlüter, Kaulfuss and Seipp (2005) Biospektrum 4: 464-5. Highly efficient reproduction of DNA in mammal cells Excellent reproducibility High and wide operating plateau No inhibition through serum Low cell toxicity Successful with different cell lines Excellent transfection results Successfully tested on a variety of cell types. siRNA: CEM, COS-7, ES-D3,HeLa, HL60, LNCaP, OVCAR3, PC3, SW620 Primary cells: pAML, pHDF, pMEF, pRHC, pSMC Cell lines: 143B, 293-T, A-293, A-431, AM-C6SC8, BHK, BV-2, C2C12, C6, CHO, CHO-DHFR, COS-1, COS-7, CRFK, Colo-205, DU-145, ECV-304, EMC, F98 (glioma), GD25-ß1, HBL 100, HCT-116, HCT-15, HEK293, HeLa, HeLa-S3, Hep-3B, Hep-G2, HOSE, HSG, HT-22, HT-29, HUVEC, JURKAT, LLC-PK1, LS174T, LoVo, MCF-7, MDCK, MeWo, MV3, NIH-3T3, NS20Y, OK, P815, PC3, PT-11, Rcho-1, SAOS, SH-SY5Y, SK-MEL-28, SKOV-3, SM10, SW-480, THP-1, tsA201, U87 (glioma), Vero 76, et al. Approx. 400 assays per ml in 6 wells. Dependent on the cell line used. Figure: Transfection of a variety of cell lines with ROTI ® Fect and other commercially available transfectants. Transfection was performed in 24wells using 0.25 µg DNA each, with 1.5 µl ROTI ® Fect or acc. to instructions given by the manufacturers. Luciferase activity was measured 48 hours post transfection. Data of cells transfected with ROTI ® Fect was set to 100 %.
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ROTI ® Fect CELLPURE ® ready-to-use, for transfection ROTI ® Fect is a liposome-based transfection reagent consisting of a mixture of polycationic and neutral lipids. Compared to monocationic lipid formulations, ROTI ® Fect has a higher efficiency with lower levels being required. Its excellent reproducibility in combination with its high level of efficiency reduced optimisation work to a minimum. Because of its low toxicity, a wide range of cell lines can be transfected efficiently. An efficient triple-cotransfection using ROTI ® Fect is described in Ufartes, Schlüter, Kaulfuss and Seipp (2005) Biospektrum 4: 464-5. Highly efficient reproduction of DNA in mammal cells Excellent reproducibility High and wide operating plateau No inhibition through serum Low cell toxicity Successful with different cell lines Excellent transfection results Successfully tested on a variety of cell types. siRNA: CEM, COS-7, ES-D3,HeLa, HL60, LNCaP, OVCAR3, PC3, SW620 Primary cells: pAML, pHDF, pMEF, pRHC, pSMC Cell lines: 143B, 293-T, A-293, A-431, AM-C6SC8, BHK, BV-2, C2C12, C6, CHO, CHO-DHFR, COS-1, COS-7, CRFK, Colo-205, DU-145, ECV-304, EMC, F98 (glioma), GD25-ß1, HBL 100, HCT-116, HCT-15, HEK293, HeLa, HeLa-S3, Hep-3B, Hep-G2, HOSE, HSG, HT-22, HT-29, HUVEC, JURKAT, LLC-PK1, LS174T, LoVo, MCF-7, MDCK, MeWo, MV3, NIH-3T3, NS20Y, OK, P815, PC3, PT-11, Rcho-1, SAOS, SH-SY5Y, SK-MEL-28, SKOV-3, SM10, SW-480, THP-1, tsA201, U87 (glioma), Vero 76, et al. Approx. 400 assays per ml in 6 wells. Dependent on the cell line used. Figure: Transfection of a variety of cell lines with ROTI ® Fect and other commercially available transfectants. Transfection was performed in 24wells using 0.25 µg DNA each, with 1.5 µl ROTI ® Fect or acc. to instructions given by the manufacturers. Luciferase activity was measured 48 hours post transfection. Data of cells transfected with ROTI ® Fect was set to 100 %.
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DOTAP CELLPURE ® ready-to-use, for transfection Purity, QC: Controlled by TLC, NMR, standard transfection test with HeLa-, CV-1-cells. At least 99 % Stability: Minimum shelf-life 6 month at +4 °C Culture media: Suitable for whole and serum-free media Formulation Transfection reagent DOTAP is an aqueous liposome formulation of N-[1-(2,3- D i o leoyloxy)]-N,N,N- t rimethyl a mmonium p ropane methyl sulphate. Concentration is approx. 1 mg/ml. Successfully tested on a variety of cell types. A375-C6, A549, B6.NOS, Balb/3T3, BalbSV, BHK, BNL CL.2, C2, C6, CaShi, CCRF-CEM/VLB, CHO, COS-1, COS-7, CV-1, D-17, DTM, EL-4, Fibroblasts, GSM06, H225, HEL, HeLa, Hepa 1-6, HepG2, HMEC-1, Hippocampal neurons, Huh7, I-3, I10, JEG-3, Jurkat, LH, Ltk, Lymphocytes, PBL, M17, MC57, MCF-7, MDA-MB-435, MEC-J, MH1C1, mhAT3F, mhAT3F, NG 108-15, NIH/3T3, PXF 1118, RASM, REF-1, Ret-1, ROS 17/2.8, SCC-13, SK-BR-3, Smooth muscle cells, ST33c, T2-Kb, U87, Vero, WI-38, Y1, et al.
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ROTI ® Insectofect CELLPURE ® ready-to-use, for transfection Recombinant proteins at a large scale can be manufactured under simple conditions in insect cell cultures with the non-human pathogenic baculovirus system and a simple scale-up procedure. ROTI ® Insectofect enables best transfection results with a wide spectrum of activity and very low toxicity. ROTI ® Insectofect consists of an aqueous solution of polycationic lipids and was specially developed for liposome mediated transfection of insect cells. Because of its special formulation, it has a much higher efficiency than standard transfectants, such as calcium phosphate or DEAE. No serum inhibition Superior transfection efficiency High and wide operating plateau Excellent reproducibility Very low cell toxicity Quick and easy handling Successfully tested on a variety of cell types. Ag55, Anso, As43, Bm5, Cpp512, IPLB-SF21, LD652, Mos-20, S2, SF9, SL2. Ideal for transfection of SF9, SL-3 and SPC-SL 52 cells. Approx. 150 assays per ml in 6 wells. Dependent on the cell line used. The figure shows evaluation of an X-Gal assay after transfection of β-Galactosidase-genes in SF9 and SPC-SL 52 cells. Not a medical device / Not an IVD product
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DEAE Dextran for biochemistry DEAE is the oldest transfection reagent known. The method of complexation of plasmid DNA with diethylaminoethyldextran has been published in 1968 by McCutchan. Positively charged DEAE binds negatively charged DNA to aggregates, which then bind to negtively charged surface structures of cell membranes. Uptake is done via endocytosis. This DEAE method needs only little amounts of DNA, while resulting in high transfection efficiency of up to 30 %. However, the cytotoxic effects caused by DEAE allow only transient transfections. The following cell lines are well transfectable: CV-1, BSC-1 and COS cells, and other stabile, insensitive cell lines. Stock solution: 50 mg/ml in H 2 O. Autoclave. End concentration: 1 mg/ml (transfection for 30-90 min.) or 250 µg/ml (transfection for up to 8 h)
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ROTI ® Fect RNAi Kit CELLPURE ® ready-to-use, for transfection New, powerful transfection reagent for liposoma mediated siRNA and miRNA transfection of all mammalian cells in culture. Liposoma formulation and enclosed buffer were specially established and designed for core parameters of RNAi: Most efficient quantitative endocytosis plus release of nucleic acids into the cytosol, without subsequent transport to the nucleus. ROTI ® Fect RNAi provides you with superior knockdown results, even using low amounts of RNA.Hereby, the ROTI ® Fect technology guarantees minimisation of toxic effects during transfection, thus making these reagents suitable for very sensitive cell lines of primary cells. The detailed instruction-for-use contains a standardised transfection protocol working via a definite RNA/liposoma ratio and providing good results starting with the very first assay - making elaborate optimisations of transfection obsolete. Evaluation of results is often possible after 48 hours, enabling two sequential assays per week. Optimised RNA release into the cytosol High levels of gene silencing with low RNA concentration Efficient knockdown after 48 hours already No elaborate optimisation necessary Very low cell toxicity Suitable for cell lines and primary cells Straightforward, efficient protocol for whole medium included Successfully tested on a variety of cell types. Primary cells: MCS, M1 Cell lines: A549, 3T3-L1, B16F10, CHO-K1, H441, HT1080, HeLa, HeLa-KB, HepG2 The kit contains: 0.2 and 1 ml, respectively, ROTI ® Fect RNAi liposoma formulation (Art. No. 2911), and 0.4 and 2 ml, respectively, ROTI ® Fect RNAi buffer (Art. No. 2913). Les composants unitaires de ce kit ne peuvent pas être achetés séparément. Approx. 650 assays per ml in 24wells. Dependend on the cell line used. Figure: Knockdown of luciferase activity in stably Luc-transfected cell lines and cells (48 wells, 2 × 10 4 cells, 30 pMol siRNA). Percent reduction in luciferase activity (measured by luminometer) in knockdown cells compared to cells transfected with control RNA. Measurement 2 days after transfection. Figure: Knockdown of luciferase activity in stably Luc-transfected cell lines and cells (48 wells, 2 × 10 4 cells, 30 pMol siRNA). Percent reduction in luciferase activity (measured by luminometer) in knockdown cells compared to cells transfected with control RNA. Comparison of results obtained using different transfection reagents. Measurement 2 days after transfection.
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ROTI ® Fect Plus CELLPURE ® ready-to-use, for transfection Highly sophisticated transfection reagent for liposoma mediated transfection of eukaryotic cell lines and primary cells in culture. ROTI ® Fect PLUS further reduces the toxic effects of transfection, making it a versatile tool for transfection of sensitive cell lines or primary cells. Based on this, ROTI ® Fect PLUS is also especially recommended for transfection prior to in vivo analyses (siRNA-Assays, in vivo life microscopy, etc.), since the transfection stress is minimized, reducing stress dependent effects like the rate of apoptosis induced cells. Additionally, the stability of nucleic acid/lipid complexes is diminished in comparison with ROTI ® Fect, therefore improving the release of nucleic acids in the cell. Thus, the newly established liposoma formulation of ROTI ® Fect PLUS guarantees gentle and highly efficient transfections, even when hard-to-transfect or particularly sensitive cell lines are handled. Particularly low cell toxicity Superior transfection efficiency with all cell lines Especially recommended for hard-to-transfect cell lines Particularly suited for use with primary cells Excellent reproducibility No serum inhibition Successfully tested on a variety of cell types. siRNA: 1BR3, HEK293, HeLa, LN-308, T98G. U87MG, UACC-257 Primary cells: 1BR3, 8VM, CGN, HMEC, HUVEC, PBMC, S15VM Cell lines: 1BRneo, 143B, 293T, NSCLC, alphaTC1, B16-F10, BHK-21, C2C12, C6, CHO, CHO-K1, Colo205, COS-7, EVLC2, HaCaT, HAEC, HCT116, HEK293, HeLa, HepG2, Hep 3B, IMR32, Jurkat, Kelly, KG1, La1-5S, LN-308, M2-10B4, MDCK, MEF, MG-63, MIN6, MLEC32, mPAC, mpkCCD, N18TG2, N2a, N-Tera2, NG NIH-3T3, Panc-1, PC 12, Phi-NX, Raji, RAW264.7, RD, RF24, RGE, S2, S91, SMA-560, SK-N-BE, SW480, T98G, U-2 OS, Vero, et al. Approx. 400 assays per ml in 6 wells. Dependent on the cell line used. Figure: Via ROTI ® Fect PLUS transfected cells. A: Fluorescent light (520 nm), B: Standard light. Above: COS7 cells (0.5 × 10 5 cells / cm 2 in 24-well). Transfection after 24 h using 0.9 μg pCMV-GFP + 3.6 μl ROTI ® Fect PLUS (ratio 1:4). Analysis after 48 h. Below: BHK cells (0.2 × 10 5 cells / cm 2 in 24-well). Transfection after 24 h using 0.3 μg pCMV-GFP + 1.2 μl ROTI ® Fect PLUS (ratio 1:4). Analysis after 48 h. Figure: Transfection efficiency subject to ratio of DNA to ROTI ® Fect PLUS. For most cell lines, the optimised composition of transfection complex is 1:3 to 1:4. Vero: 1.2 × 10 5 cells / cm 2 ; MDCK: 1.2 × 10 5 cells / cm 2 ; COS7: 5 × 10 4 cells / cm 2 ; HeLa: 1 × 10 5 cells / cm 2 . Transfection with pCMV-LacZ 24 h after sawing in 48 wells in serum-free DMEM. Analysis (ONPG assay) after 48 h. Figure: Transfection efficiency of ROTI ® Fect PLUS given in percentage. For standardisation, transfection efficiency of ROTI ® Fect was set as reference (100 %). Compared to ROTI ® Fect, transfection efficiency achieved by ROTI ® Fect PLUS is at least equivalent. For some cell lines known to be hard-to-transfect with ROTI ® Fect, e.g. Vero, CV1, or CHO-K1 cells, ROTI ® Fect PLUS achieves multiple enhancement of transfection efficiency.
Product Detail
DEAE Dextran for biochemistry DEAE is the oldest transfection reagent known. The method of complexation of plasmid DNA with diethylaminoethyldextran has been published in 1968 by McCutchan. Positively charged DEAE binds negatively charged DNA to aggregates, which then bind to negtively charged surface structures of cell membranes. Uptake is done via endocytosis. This DEAE method needs only little amounts of DNA, while resulting in high transfection efficiency of up to 30 %. However, the cytotoxic effects caused by DEAE allow only transient transfections. The following cell lines are well transfectable: CV-1, BSC-1 and COS cells, and other stabile, insensitive cell lines. Stock solution: 50 mg/ml in H 2 O. Autoclave. End concentration: 1 mg/ml (transfection for 30-90 min.) or 250 µg/ml (transfection for up to 8 h)
Product Detail
DOTAP CELLPURE ® ready-to-use, for transfection Purity, QC: Controlled by TLC, NMR, standard transfection test with HeLa-, CV-1-cells. At least 99 % Stability: Minimum shelf-life 6 month at +4 °C Culture media: Suitable for whole and serum-free media Formulation Transfection reagent DOTAP is an aqueous liposome formulation of N-[1-(2,3- D i o leoyloxy)]-N,N,N- t rimethyl a mmonium p ropane methyl sulphate. Concentration is approx. 1 mg/ml. Successfully tested on a variety of cell types. A375-C6, A549, B6.NOS, Balb/3T3, BalbSV, BHK, BNL CL.2, C2, C6, CaShi, CCRF-CEM/VLB, CHO, COS-1, COS-7, CV-1, D-17, DTM, EL-4, Fibroblasts, GSM06, H225, HEL, HeLa, Hepa 1-6, HepG2, HMEC-1, Hippocampal neurons, Huh7, I-3, I10, JEG-3, Jurkat, LH, Ltk, Lymphocytes, PBL, M17, MC57, MCF-7, MDA-MB-435, MEC-J, MH1C1, mhAT3F, mhAT3F, NG 108-15, NIH/3T3, PXF 1118, RASM, REF-1, Ret-1, ROS 17/2.8, SCC-13, SK-BR-3, Smooth muscle cells, ST33c, T2-Kb, U87, Vero, WI-38, Y1, et al.
Product Detail
ROTI ® Fect CELLPURE ® ready-to-use, for transfection ROTI ® Fect is a liposome-based transfection reagent consisting of a mixture of polycationic and neutral lipids. Compared to monocationic lipid formulations, ROTI ® Fect has a higher efficiency with lower levels being required. Its excellent reproducibility in combination with its high level of efficiency reduced optimisation work to a minimum. Because of its low toxicity, a wide range of cell lines can be transfected efficiently. An efficient triple-cotransfection using ROTI ® Fect is described in Ufartes, Schlüter, Kaulfuss and Seipp (2005) Biospektrum 4: 464-5. Highly efficient reproduction of DNA in mammal cells Excellent reproducibility High and wide operating plateau No inhibition through serum Low cell toxicity Successful with different cell lines Excellent transfection results Successfully tested on a variety of cell types. siRNA: CEM, COS-7, ES-D3,HeLa, HL60, LNCaP, OVCAR3, PC3, SW620 Primary cells: pAML, pHDF, pMEF, pRHC, pSMC Cell lines: 143B, 293-T, A-293, A-431, AM-C6SC8, BHK, BV-2, C2C12, C6, CHO, CHO-DHFR, COS-1, COS-7, CRFK, Colo-205, DU-145, ECV-304, EMC, F98 (glioma), GD25-ß1, HBL 100, HCT-116, HCT-15, HEK293, HeLa, HeLa-S3, Hep-3B, Hep-G2, HOSE, HSG, HT-22, HT-29, HUVEC, JURKAT, LLC-PK1, LS174T, LoVo, MCF-7, MDCK, MeWo, MV3, NIH-3T3, NS20Y, OK, P815, PC3, PT-11, Rcho-1, SAOS, SH-SY5Y, SK-MEL-28, SKOV-3, SM10, SW-480, THP-1, tsA201, U87 (glioma), Vero 76, et al. Approx. 400 assays per ml in 6 wells. Dependent on the cell line used. Figure: Transfection of a variety of cell lines with ROTI ® Fect and other commercially available transfectants. Transfection was performed in 24wells using 0.25 µg DNA each, with 1.5 µl ROTI ® Fect or acc. to instructions given by the manufacturers. Luciferase activity was measured 48 hours post transfection. Data of cells transfected with ROTI ® Fect was set to 100 %.
Product Detail
ROTI ® Fect RNAi Kit CELLPURE ® ready-to-use, for transfection New, powerful transfection reagent for liposoma mediated siRNA and miRNA transfection of all mammalian cells in culture. Liposoma formulation and enclosed buffer were specially established and designed for core parameters of RNAi: Most efficient quantitative endocytosis plus release of nucleic acids into the cytosol, without subsequent transport to the nucleus. ROTI ® Fect RNAi provides you with superior knockdown results, even using low amounts of RNA.Hereby, the ROTI ® Fect technology guarantees minimisation of toxic effects during transfection, thus making these reagents suitable for very sensitive cell lines of primary cells. The detailed instruction-for-use contains a standardised transfection protocol working via a definite RNA/liposoma ratio and providing good results starting with the very first assay - making elaborate optimisations of transfection obsolete. Evaluation of results is often possible after 48 hours, enabling two sequential assays per week. Optimised RNA release into the cytosol High levels of gene silencing with low RNA concentration Efficient knockdown after 48 hours already No elaborate optimisation necessary Very low cell toxicity Suitable for cell lines and primary cells Straightforward, efficient protocol for whole medium included Successfully tested on a variety of cell types. Primary cells: MCS, M1 Cell lines: A549, 3T3-L1, B16F10, CHO-K1, H441, HT1080, HeLa, HeLa-KB, HepG2 The kit contains: 0.2 and 1 ml, respectively, ROTI ® Fect RNAi liposoma formulation (Art. No. 2911), and 0.4 and 2 ml, respectively, ROTI ® Fect RNAi buffer (Art. No. 2913). Les composants unitaires de ce kit ne peuvent pas être achetés séparément. Approx. 650 assays per ml in 24wells. Dependend on the cell line used. Figure: Knockdown of luciferase activity in stably Luc-transfected cell lines and cells (48 wells, 2 × 10 4 cells, 30 pMol siRNA). Percent reduction in luciferase activity (measured by luminometer) in knockdown cells compared to cells transfected with control RNA. Measurement 2 days after transfection. Figure: Knockdown of luciferase activity in stably Luc-transfected cell lines and cells (48 wells, 2 × 10 4 cells, 30 pMol siRNA). Percent reduction in luciferase activity (measured by luminometer) in knockdown cells compared to cells transfected with control RNA. Comparison of results obtained using different transfection reagents. Measurement 2 days after transfection.
Product Detail
ROTI ® Fect Plus CELLPURE ® ready-to-use, for transfection Highly sophisticated transfection reagent for liposoma mediated transfection of eukaryotic cell lines and primary cells in culture. ROTI ® Fect PLUS further reduces the toxic effects of transfection, making it a versatile tool for transfection of sensitive cell lines or primary cells. Based on this, ROTI ® Fect PLUS is also especially recommended for transfection prior to in vivo analyses (siRNA-Assays, in vivo life microscopy, etc.), since the transfection stress is minimized, reducing stress dependent effects like the rate of apoptosis induced cells. Additionally, the stability of nucleic acid/lipid complexes is diminished in comparison with ROTI ® Fect, therefore improving the release of nucleic acids in the cell. Thus, the newly established liposoma formulation of ROTI ® Fect PLUS guarantees gentle and highly efficient transfections, even when hard-to-transfect or particularly sensitive cell lines are handled. Particularly low cell toxicity Superior transfection efficiency with all cell lines Especially recommended for hard-to-transfect cell lines Particularly suited for use with primary cells Excellent reproducibility No serum inhibition Successfully tested on a variety of cell types. siRNA: 1BR3, HEK293, HeLa, LN-308, T98G. U87MG, UACC-257 Primary cells: 1BR3, 8VM, CGN, HMEC, HUVEC, PBMC, S15VM Cell lines: 1BRneo, 143B, 293T, NSCLC, alphaTC1, B16-F10, BHK-21, C2C12, C6, CHO, CHO-K1, Colo205, COS-7, EVLC2, HaCaT, HAEC, HCT116, HEK293, HeLa, HepG2, Hep 3B, IMR32, Jurkat, Kelly, KG1, La1-5S, LN-308, M2-10B4, MDCK, MEF, MG-63, MIN6, MLEC32, mPAC, mpkCCD, N18TG2, N2a, N-Tera2, NG NIH-3T3, Panc-1, PC 12, Phi-NX, Raji, RAW264.7, RD, RF24, RGE, S2, S91, SMA-560, SK-N-BE, SW480, T98G, U-2 OS, Vero, et al. Approx. 400 assays per ml in 6 wells. Dependent on the cell line used. Figure: Via ROTI ® Fect PLUS transfected cells. A: Fluorescent light (520 nm), B: Standard light. Above: COS7 cells (0.5 × 10 5 cells / cm 2 in 24-well). Transfection after 24 h using 0.9 μg pCMV-GFP + 3.6 μl ROTI ® Fect PLUS (ratio 1:4). Analysis after 48 h. Below: BHK cells (0.2 × 10 5 cells / cm 2 in 24-well). Transfection after 24 h using 0.3 μg pCMV-GFP + 1.2 μl ROTI ® Fect PLUS (ratio 1:4). Analysis after 48 h. Figure: Transfection efficiency subject to ratio of DNA to ROTI ® Fect PLUS. For most cell lines, the optimised composition of transfection complex is 1:3 to 1:4. Vero: 1.2 × 10 5 cells / cm 2 ; MDCK: 1.2 × 10 5 cells / cm 2 ; COS7: 5 × 10 4 cells / cm 2 ; HeLa: 1 × 10 5 cells / cm 2 . Transfection with pCMV-LacZ 24 h after sawing in 48 wells in serum-free DMEM. Analysis (ONPG assay) after 48 h. Figure: Transfection efficiency of ROTI ® Fect PLUS given in percentage. For standardisation, transfection efficiency of ROTI ® Fect was set as reference (100 %). Compared to ROTI ® Fect, transfection efficiency achieved by ROTI ® Fect PLUS is at least equivalent. For some cell lines known to be hard-to-transfect with ROTI ® Fect, e.g. Vero, CV1, or CHO-K1 cells, ROTI ® Fect PLUS achieves multiple enhancement of transfection efficiency.
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
