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For purification and concentration of biomolecules and for purification of solutions. Type: ROTI ® Spin MINI: 1.5 ml sample tube for up to 500 µl sample ROTI ® Spin MIDI: 15 ml sample tube for up to 5 ml sample ROTI ® Spin MAXI: 50 ml sample tube for up to 15 ml sample Centrifugal filters for ultrafiltration of biomolecules. The membranes made of highly chemical-resistant polyethersulfone have been tightly sealed to prevent leakage and loss of samples. Furthermore, the membranes underwent special treatment minimising the binding of biomolecules, in order to optimise sample recovery rates. ROTI ® Spin Centrifugal filters can be applied to a variety of applications and can be used to replace tedious and labour-intensive methods like gel filtration, gel purification or precipitation. Application is time-saving and very simple - only few centrifugal steps are necessary to concentrate and recover your sample. During this, the sample remains untouched in the upper reservoir, and cross-contamination is efficiently prevented even when large numbers of samples are handled. Application examples Desalting or buffer exchange following restriction digest, PCR or such; separation of free nucleotides and polymerised DNA; removal of contaminating compounds (like DNA) from stock solutions (e.g. PCR-buffers); concentration of DNA or proteins; separation of proteins of different size; sample preparation prior to HPLC. Overview for selecting the appropriate pore size MWCO Molecule size DNA size 10 kDa 30 – 90 kDa 50 – 200 bp 30 kDa 90 – 180 kDa 200 – 500 bp 50 kDa 150 – 300 kDa 500 bp – 1 kb 100 kDa >300 kDa >1 kb This product is non-sterile. It can be disinfected and sterilized by filtering 70% ethanol through the equipment. ROTI ® Spin MINI Directions for use Sample volumes: Initial volume: 50-500 μl Final volume: 10-20 μl MWCO: Centrifugation units with membranes in 5 different molecular weight cut-offs (MWCO) are available for specific applications. Select the appropriate MWCO according to the following rule of thumb: Purification of proteins/peptides: MWCO ≤1/3 of the sample molecule size. Purification of nucleic acids: MWCO ≤ 1 / 2 of the sample molecule size. General properties: Suitable for nucleic acids, proteins/peptides, other biomolecules >200 kDa, and very small bioparticles Replaces standard applications such as precipitation, dialysis, gel filtration, gel purification, column chromatography, gradient centrifugation, etc. Gentle: no shearing of DNA up to 100 kb, proteins remain native and enzymatically active Can be used without restriction on labeled samples (isotope, fluorescence, chromogenic labeling) Very high recovery rates: with a suitable MWCO, the yield is >90%. Retention rate (%) of ROTI ® Spin MINI compared to a reference product. Ultrafiltration tubes with MWCO from 3 kDa to 100 kDa with corresponding proteins were tested. Recovery rate (%) of ROTI ® Spin MINI compared to a reference product. Ultrafiltration tubes with MWCO from 3 kDa to 100 kDa with corresponding proteins were tested. Not a medical device / Not an IVD product
Product Detail
Non-sterile Centrifuge filter units for sample volumes of no more than 0.5 or 2.0 ml. For use in fixed-angle rotors for 1.5 or 15 ml sample tubes. Filter unit and collector tube are made of PP. Hydrophilic Durapore ® PVDF-membrane: extremely low protein binding. Suitable for removal of particulate matter and precipitate from aqueous samples, and for protein and nucleic acid applications Hydrophilic PTFE membrane: for removal of precipitate from solvent-based samples Procedure: Open the attached lid of the filter unit Fill the insert with the volume of sample Close the filtration unit and insert it in the centrifuge On completion of centrifuging, remove the filtration unit, open the lid and remove the insert The insert contains the residue and the sealable housing contains the filtrate Centrifuge filter unit Ultrafree Ultrafree-MC: PVDF membrane, sample volume up to 0.5 ml Filter insert volume max. 0.5 ml, collector tube volume 1.5 ml.
Product Detail
For purification and concentration of biomolecules and for purification of solutions. Type: ROTI ® Spin MINI: 1.5 ml sample tube for up to 500 µl sample ROTI ® Spin MIDI: 15 ml sample tube for up to 5 ml sample ROTI ® Spin MAXI: 50 ml sample tube for up to 15 ml sample Centrifugal filters for ultrafiltration of biomolecules. The membranes made of highly chemical-resistant polyethersulfone have been tightly sealed to prevent leakage and loss of samples. Furthermore, the membranes underwent special treatment minimising the binding of biomolecules, in order to optimise sample recovery rates. ROTI ® Spin Centrifugal filters can be applied to a variety of applications and can be used to replace tedious and labour-intensive methods like gel filtration, gel purification or precipitation. Application is time-saving and very simple - only few centrifugal steps are necessary to concentrate and recover your sample. During this, the sample remains untouched in the upper reservoir, and cross-contamination is efficiently prevented even when large numbers of samples are handled. Application examples Desalting or buffer exchange following restriction digest, PCR or such; separation of free nucleotides and polymerised DNA; removal of contaminating compounds (like DNA) from stock solutions (e.g. PCR-buffers); concentration of DNA or proteins; separation of proteins of different size; sample preparation prior to HPLC. Overview for selecting the appropriate pore size MWCO Molecule size DNA size 10 kDa 30 – 90 kDa 50 – 200 bp 30 kDa 90 – 180 kDa 200 – 500 bp 50 kDa 150 – 300 kDa 500 bp – 1 kb 100 kDa >300 kDa >1 kb This product is non-sterile. It can be disinfected and sterilized by filtering 70% ethanol through the equipment. ROTI ® Spin MINI Directions for use Sample volumes: Initial volume: 50-500 μl Final volume: 10-20 μl MWCO: Centrifugation units with membranes in 5 different molecular weight cut-offs (MWCO) are available for specific applications. Select the appropriate MWCO according to the following rule of thumb: Purification of proteins/peptides: MWCO ≤1/3 of the sample molecule size. Purification of nucleic acids: MWCO ≤ 1 / 2 of the sample molecule size. General properties: Suitable for nucleic acids, proteins/peptides, other biomolecules >200 kDa, and very small bioparticles Replaces standard applications such as precipitation, dialysis, gel filtration, gel purification, column chromatography, gradient centrifugation, etc. Gentle: no shearing of DNA up to 100 kb, proteins remain native and enzymatically active Can be used without restriction on labeled samples (isotope, fluorescence, chromogenic labeling) Very high recovery rates: with a suitable MWCO, the yield is >90%. Retention rate (%) of ROTI ® Spin MINI compared to a reference product. Ultrafiltration tubes with MWCO from 3 kDa to 100 kDa with corresponding proteins were tested. Recovery rate (%) of ROTI ® Spin MINI compared to a reference product. Ultrafiltration tubes with MWCO from 3 kDa to 100 kDa with corresponding proteins were tested. Not a medical device / Not an IVD product
Product Detail
For purification and concentration of biomolecules and for purification of solutions. Type: ROTI ® Spin MINI: 1.5 ml sample tube for up to 500 µl sample ROTI ® Spin MIDI: 15 ml sample tube for up to 5 ml sample ROTI ® Spin MAXI: 50 ml sample tube for up to 15 ml sample Centrifugal filters for ultrafiltration of biomolecules. The membranes made of highly chemical-resistant polyethersulfone have been tightly sealed to prevent leakage and loss of samples. Furthermore, the membranes underwent special treatment minimising the binding of biomolecules, in order to optimise sample recovery rates. ROTI ® Spin Centrifugal filters can be applied to a variety of applications and can be used to replace tedious and labour-intensive methods like gel filtration, gel purification or precipitation. Application is time-saving and very simple - only few centrifugal steps are necessary to concentrate and recover your sample. During this, the sample remains untouched in the upper reservoir, and cross-contamination is efficiently prevented even when large numbers of samples are handled. Application examples Desalting or buffer exchange following restriction digest, PCR or such; separation of free nucleotides and polymerised DNA; removal of contaminating compounds (like DNA) from stock solutions (e.g. PCR-buffers); concentration of DNA or proteins; separation of proteins of different size; sample preparation prior to HPLC. Overview for selecting the appropriate pore size MWCO Molecule size DNA size 10 kDa 30 – 90 kDa 50 – 200 bp 30 kDa 90 – 180 kDa 200 – 500 bp 50 kDa 150 – 300 kDa 500 bp – 1 kb 100 kDa >300 kDa >1 kb This product is non-sterile. It can be disinfected and sterilized by filtering 70% ethanol through the equipment. ROTI ® Spin MINI Directions for use Sample volumes: Initial volume: 50-500 μl Final volume: 10-20 μl MWCO: Centrifugation units with membranes in 5 different molecular weight cut-offs (MWCO) are available for specific applications. Select the appropriate MWCO according to the following rule of thumb: Purification of proteins/peptides: MWCO ≤1/3 of the sample molecule size. Purification of nucleic acids: MWCO ≤ 1 / 2 of the sample molecule size. General properties: Suitable for nucleic acids, proteins/peptides, other biomolecules >200 kDa, and very small bioparticles Replaces standard applications such as precipitation, dialysis, gel filtration, gel purification, column chromatography, gradient centrifugation, etc. Gentle: no shearing of DNA up to 100 kb, proteins remain native and enzymatically active Can be used without restriction on labeled samples (isotope, fluorescence, chromogenic labeling) Very high recovery rates: with a suitable MWCO, the yield is >90%. Retention rate (%) of ROTI ® Spin MINI compared to a reference product. Ultrafiltration tubes with MWCO from 3 kDa to 100 kDa with corresponding proteins were tested. Recovery rate (%) of ROTI ® Spin MINI compared to a reference product. Ultrafiltration tubes with MWCO from 3 kDa to 100 kDa with corresponding proteins were tested. Not a medical device / Not an IVD product
Product Detail
Non-sterile Centrifuge filter units for sample volumes of no more than 0.5 or 2.0 ml. For use in fixed-angle rotors for 1.5 or 15 ml sample tubes. Filter unit and collector tube are made of PP. Hydrophilic Durapore ® PVDF-membrane: extremely low protein binding. Suitable for removal of particulate matter and precipitate from aqueous samples, and for protein and nucleic acid applications Hydrophilic PTFE membrane: for removal of precipitate from solvent-based samples Procedure: Open the attached lid of the filter unit Fill the insert with the volume of sample Close the filtration unit and insert it in the centrifuge On completion of centrifuging, remove the filtration unit, open the lid and remove the insert The insert contains the residue and the sealable housing contains the filtrate Centrifuge filter unit Ultrafree-CL: hydrophilic PTFE membrane, sample volume up to 2.0 ml Filter insert volume max. 2 ml, collector tube volume 5 ml.
Product Detail
For purification and concentration of biomolecules and for purification of solutions. Type: ROTI ® Spin MINI: 1.5 ml sample tube for up to 500 µl sample ROTI ® Spin MIDI: 15 ml sample tube for up to 5 ml sample ROTI ® Spin MAXI: 50 ml sample tube for up to 15 ml sample Centrifugal filters for ultrafiltration of biomolecules. The membranes made of highly chemical-resistant polyethersulfone have been tightly sealed to prevent leakage and loss of samples. Furthermore, the membranes underwent special treatment minimising the binding of biomolecules, in order to optimise sample recovery rates. ROTI ® Spin Centrifugal filters can be applied to a variety of applications and can be used to replace tedious and labour-intensive methods like gel filtration, gel purification or precipitation. Application is time-saving and very simple - only few centrifugal steps are necessary to concentrate and recover your sample. During this, the sample remains untouched in the upper reservoir, and cross-contamination is efficiently prevented even when large numbers of samples are handled. Application examples Desalting or buffer exchange following restriction digest, PCR or such; separation of free nucleotides and polymerised DNA; removal of contaminating compounds (like DNA) from stock solutions (e.g. PCR-buffers); concentration of DNA or proteins; separation of proteins of different size; sample preparation prior to HPLC. Overview for selecting the appropriate pore size MWCO Molecule size DNA size 10 kDa 30 – 90 kDa 50 – 200 bp 30 kDa 90 – 180 kDa 200 – 500 bp 50 kDa 150 – 300 kDa 500 bp – 1 kb 100 kDa >300 kDa >1 kb ROTI ® Spin MIDI Directions for use Sample volumes: I nitial volume: max. 15 ml Final volume: 200 µl MWCO: Centrifugation units with membranes in 4 different molecular weight cut-offs (MWCO) are available for specific applications. Select the appropriate MWCO according to the following rule: Purification of proteins/peptides: MWCO ≤1/3 of the sample molecule size. Purification of nucleic acids: MWCO ≤ 1 / 2 of the sample molecule size. General properties: Suitable for nucleic acids, proteins/peptides, other biomolecules >200 kDa and very small bioparticles Replaces standard applications such as precipitation, dialysis, gel filtration, gel purification, column chromatography, gradient centrifugation, etc. Gentle: no shearing of DNA up to 100 kb, proteins remain native and enzymatically active Can be used without restriction for labelled samples (isotope, fluorescence, chromogenic labelling) Very high recovery rates: with the right MWCO, the yield is >90%" Not a medical device / Not an IVD product
Product Detail
Material: PP, modified acrylic (MBS). Sterile For filtration of sample volumes up to 50 ml. Millipore Express ® PES membrane with high flow rate and low protein binding: for routine sterile filtration of tissue culture media, sera, buffers and other aqueous solutions Durapore ® PVDF membrane with very low protein binding: for sterile and clear filtration of aqueous solutions Hydrophilic nylon mesh filter: for removing coarse particulate matter, e.g. for applications with stem, heart muscle and nerve cells, or skeletal tissue Procedure: Connect the filter unit to the centrifuge tube containing the sample. Rotate the filter unit through 180° and apply vacuum.Optional: screw the funnel attachment onto the sterile filter unit, fill with the sample and apply vacuum, Steriflip ® filter unit filters directly into a sterile 50 ml centrifuge tube. Vacuum filter unit Steriflip ® Delivery incl. fitted 50 ml centrifuge tube and lid (each packaged separately) and a single tube holder (blue). Delivery without funnel attachment or second 50 ml centrifuge tube (e.g. Art. No. N462.1), please order separately.
Product Detail
For purification and concentration of biomolecules and for purification of solutions. Type: ROTI ® Spin MINI: 1.5 ml sample tube for up to 500 µl sample ROTI ® Spin MIDI: 15 ml sample tube for up to 5 ml sample ROTI ® Spin MAXI: 50 ml sample tube for up to 15 ml sample Centrifugal filters for ultrafiltration of biomolecules. The membranes made of highly chemical-resistant polyethersulfone have been tightly sealed to prevent leakage and loss of samples. Furthermore, the membranes underwent special treatment minimising the binding of biomolecules, in order to optimise sample recovery rates. ROTI ® Spin Centrifugal filters can be applied to a variety of applications and can be used to replace tedious and labour-intensive methods like gel filtration, gel purification or precipitation. Application is time-saving and very simple - only few centrifugal steps are necessary to concentrate and recover your sample. During this, the sample remains untouched in the upper reservoir, and cross-contamination is efficiently prevented even when large numbers of samples are handled. Application examples Desalting or buffer exchange following restriction digest, PCR or such; separation of free nucleotides and polymerised DNA; removal of contaminating compounds (like DNA) from stock solutions (e.g. PCR-buffers); concentration of DNA or proteins; separation of proteins of different size; sample preparation prior to HPLC. Overview for selecting the appropriate pore size MWCO Molecule size DNA size 10 kDa 30 – 90 kDa 50 – 200 bp 30 kDa 90 – 180 kDa 200 – 500 bp 50 kDa 150 – 300 kDa 500 bp – 1 kb 100 kDa >300 kDa >1 kb ROTI ® Spin MIDI Directions for use Sample volumes: I nitial volume: max. 15 ml Final volume: 200 µl MWCO: Centrifugation units with membranes in 4 different molecular weight cut-offs (MWCO) are available for specific applications. Select the appropriate MWCO according to the following rule: Purification of proteins/peptides: MWCO ≤1/3 of the sample molecule size. Purification of nucleic acids: MWCO ≤ 1 / 2 of the sample molecule size. General properties: Suitable for nucleic acids, proteins/peptides, other biomolecules >200 kDa and very small bioparticles Replaces standard applications such as precipitation, dialysis, gel filtration, gel purification, column chromatography, gradient centrifugation, etc. Gentle: no shearing of DNA up to 100 kb, proteins remain native and enzymatically active Can be used without restriction for labelled samples (isotope, fluorescence, chromogenic labelling) Very high recovery rates: with the right MWCO, the yield is >90%" Not a medical device / Not an IVD product
Product Detail
for molecular biology For easy and speedy extraction of DNA fragments from agarose gels. Extraction in 10 minutes High recovery of fully intact DNA Highly efficient with all gel running buffers Easy application - even during student courses Convenient handling even of large sample numbers Recovery of functionally intact DNA in one spin-step. The agarose slice is placed directly into the pre-assembled device and centrifuged for 10 minutes (fig. B). Melting or enzymatic digestion is not required. The agarose slice is nebulised and the DNA is filtered and purified during centrifugation. The eluate may simply be stored away in the extraction tube or collected from the tube in order to apply the DNA to subsequent analyses. Directions for use Compatible with all agaroses with standard gelling temperature (not compatible with Low-Melting agarose). Suitable for fragments of 100 bp up to 10,000 bp. Ideal also for labelled DNA, e.g. after fluorescence- or radioactive labelling. Reliable recovery rates for fragments of 100 bp up to 2,000 bp. The gel extraction units may be used with all standard gel running buffers. Following elution from gels run with ROTIPHORESE ® 10x TAE buffer light (Art. No. 0122.1), the DNA may be applied directly to cloning or sequencing. Compatible with all standard Mini-centrifuges. One extraction unit consists of a 1,5 ml standard reaction tube and a removable nebuliser/filter unit. Gel extraction kit Ultrafree ® DA The kit contains 50 standard reaction vessels and 50 removable nebulizer/filter units
Product Detail
For purification and concentration of biomolecules and for purification of solutions. Type: ROTI ® Spin MINI: 1.5 ml sample tube for up to 500 µl sample ROTI ® Spin MIDI: 15 ml sample tube for up to 5 ml sample ROTI ® Spin MAXI: 50 ml sample tube for up to 15 ml sample Centrifugal filters for ultrafiltration of biomolecules. The membranes made of highly chemical-resistant polyethersulfone have been tightly sealed to prevent leakage and loss of samples. Furthermore, the membranes underwent special treatment minimising the binding of biomolecules, in order to optimise sample recovery rates. ROTI ® Spin Centrifugal filters can be applied to a variety of applications and can be used to replace tedious and labour-intensive methods like gel filtration, gel purification or precipitation. Application is time-saving and very simple - only few centrifugal steps are necessary to concentrate and recover your sample. During this, the sample remains untouched in the upper reservoir, and cross-contamination is efficiently prevented even when large numbers of samples are handled. Application examples Desalting or buffer exchange following restriction digest, PCR or such; separation of free nucleotides and polymerised DNA; removal of contaminating compounds (like DNA) from stock solutions (e.g. PCR-buffers); concentration of DNA or proteins; separation of proteins of different size; sample preparation prior to HPLC. Overview for selecting the appropriate pore size MWCO Molecule size DNA size 10 kDa 30 – 90 kDa 50 – 200 bp 30 kDa 90 – 180 kDa 200 – 500 bp 50 kDa 150 – 300 kDa 500 bp – 1 kb 100 kDa >300 kDa >1 kb ROTI ® Spin MAXI Directions for use Sample volumes: I nitial volume: max. 15 ml Final volume: 200 µl MWCO: Centrifugation units with membranes in 4 different molecular weight cut-offs (MWCO) are available for specific applications. Select the appropriate MWCO according to the following rule: Purification of proteins/peptides: MWCO ≤1/3 of the sample molecule size. Purification of nucleic acids: MWCO ≤ 1 / 2 of the sample molecule size. General properties: Suitable for nucleic acids, proteins/peptides, other biomolecules >200 kDa and very small bioparticles Replaces standard applications such as precipitation, dialysis, gel filtration, gel purification, column chromatography, gradient centrifugation, etc. Gentle: no shearing of DNA up to 100 kb, proteins remain native and enzymatically active Can be used without restriction for labelled samples (isotope, fluorescence, chromogenic labelling) Very high recovery rates: with the right MWCO, the yield is >90%" Not a medical device / Not an IVD product
Product Detail
For purification and concentration of biomolecules and for purification of solutions. Type: ROTI ® Spin MINI: 1.5 ml sample tube for up to 500 µl sample ROTI ® Spin MIDI: 15 ml sample tube for up to 5 ml sample ROTI ® Spin MAXI: 50 ml sample tube for up to 15 ml sample Centrifugal filters for ultrafiltration of biomolecules. The membranes made of highly chemical-resistant polyethersulfone have been tightly sealed to prevent leakage and loss of samples. Furthermore, the membranes underwent special treatment minimising the binding of biomolecules, in order to optimise sample recovery rates. ROTI ® Spin Centrifugal filters can be applied to a variety of applications and can be used to replace tedious and labour-intensive methods like gel filtration, gel purification or precipitation. Application is time-saving and very simple - only few centrifugal steps are necessary to concentrate and recover your sample. During this, the sample remains untouched in the upper reservoir, and cross-contamination is efficiently prevented even when large numbers of samples are handled. Application examples Desalting or buffer exchange following restriction digest, PCR or such; separation of free nucleotides and polymerised DNA; removal of contaminating compounds (like DNA) from stock solutions (e.g. PCR-buffers); concentration of DNA or proteins; separation of proteins of different size; sample preparation prior to HPLC. Overview for selecting the appropriate pore size MWCO Molecule size DNA size 10 kDa 30 – 90 kDa 50 – 200 bp 30 kDa 90 – 180 kDa 200 – 500 bp 50 kDa 150 – 300 kDa 500 bp – 1 kb 100 kDa >300 kDa >1 kb This product is non-sterile. It can be disinfected and sterilized by filtering 70% ethanol through the equipment. ROTI ® Spin MINI Directions for use Sample volumes: Initial volume: 50-500 μl Final volume: 10-20 μl MWCO: Centrifugation units with membranes in 5 different molecular weight cut-offs (MWCO) are available for specific applications. Select the appropriate MWCO according to the following rule of thumb: Purification of proteins/peptides: MWCO ≤1/3 of the sample molecule size. Purification of nucleic acids: MWCO ≤ 1 / 2 of the sample molecule size. General properties: Suitable for nucleic acids, proteins/peptides, other biomolecules >200 kDa, and very small bioparticles Replaces standard applications such as precipitation, dialysis, gel filtration, gel purification, column chromatography, gradient centrifugation, etc. Gentle: no shearing of DNA up to 100 kb, proteins remain native and enzymatically active Can be used without restriction on labeled samples (isotope, fluorescence, chromogenic labeling) Very high recovery rates: with a suitable MWCO, the yield is >90%. Retention rate (%) of ROTI ® Spin MINI compared to a reference product. Ultrafiltration tubes with MWCO from 3 kDa to 100 kDa with corresponding proteins were tested. Recovery rate (%) of ROTI ® Spin MINI compared to a reference product. Ultrafiltration tubes with MWCO from 3 kDa to 100 kDa with corresponding proteins were tested. Not a medical device / Not an IVD product
Product Detail
Non-sterile Centrifuge filter units for sample volumes of no more than 0.5 or 2.0 ml. For use in fixed-angle rotors for 1.5 or 15 ml sample tubes. Filter unit and collector tube are made of PP. Hydrophilic Durapore ® PVDF-membrane: extremely low protein binding. Suitable for removal of particulate matter and precipitate from aqueous samples, and for protein and nucleic acid applications Hydrophilic PTFE membrane: for removal of precipitate from solvent-based samples Procedure: Open the attached lid of the filter unit Fill the insert with the volume of sample Close the filtration unit and insert it in the centrifuge On completion of centrifuging, remove the filtration unit, open the lid and remove the insert The insert contains the residue and the sealable housing contains the filtrate Centrifuge filter unit Ultrafree-CL: hydrophilic PTFE membrane, sample volume up to 2.0 ml Filter insert volume max. 2 ml, collector tube volume 5 ml.
Product Detail© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
