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Buffer solution for preparation of antibody incubation solutions and stabile antibody dilutions for storage. With green dye for optimum handling or as a clear solution. Enhances signal strength Reduces background Very easy application Compatible with paraffin- and cryo sections Due to the particularly designed formulation, antigen/antibody binding is enhanced, while reducing background signals in parallel (Grube (2004) Arch Histol Cytol. 67:115-34 and Buchwalow et al. (2011) Sci Rep. 1:28). Stability of the antibodies is significantly enhanced. Directions for use The buffer solution is suitable for dilution of all primary and secondary antibodies, all mono- and polyclonals, as well as for all labelled as well as non-labelled antibodies. The antibodies are just diluted in the ready-to-use diluent and applied to the slides after blocking. For reduction of the amount needed and for optimised use of high-priced antibodies we recommend to use the ROTI ® Liquid Barrier Marker (red, Art. No. AN92, or colourless, Art. No. AN91), in order to prepare tiny incubation chambers on top of the slides. Antibody Diluent clear ready-to-use, for immunohistochemistry Not a medical device / Not an IVD product
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Kit for amplification of immunochemical detection signals on blot membranes and in ELISA. Strong enhancement of signal strength Most easy application The Signal Enhancer Western/ELISA enhances antigen-antibody reactions in a variety of immunoassays such as Western blotting, dot blotting and ELISA. It can significantly enhance detection of weak immunoreactive and low abundance target proteins and is well compatible with all common assay versions, membranes and ELISA plates. Signal enhancement is dependent on several parameters such as the particular antigen/antibody pair, the particular detection enzyme, the substrate chosen and so on. In most cases, however, the signal strength is about 5 to 10fold higher than with the substrate alone. Compatible with: Western and ELISA HRP and AP all common membranes all common substrates For application, the two solutions Signal Enhancer Western/ELISA-1 and -2 are simply used as buffer solutions for preparation of the primary- and secondary antibody incubation solutions. Following incubation and washing, all common detection procedures may be applied, for instance colour staining with ROTI ® DAB-Kit (Art. No. 9202) or chemoluminescent staining with ROTI ® Lumin plus (Art. No. 3692). Signal Enhancer Western/ELISA-2 for immunochemistry The solution Signal Enhancer Western/ELISA-2 enhances binding of secondary antibodies in a variety of immunoassays such as Western blotting, dot blotting and ELISA. Even for weak immunoreactive and low abundance target proteins strong signals may thus be achieved. The solution is well compatible with all common assay versions, membranes and ELISA plates. Recommended for performance of assays using one antibody (labelled) only. Directions for use For application, the solution Signal Enhancer Western/ELISA-2 is simply used as buffer solutions for preparation of the secondary antibody incubation solution. Following incubation and washing, all common detection procedures may be applied, for instance colour staining with ROTI ® DAB-Kit (Art. No. 9202) or chemoluminescent staining with ROTI ® Lumin plus.
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DAB metal enhancer is used to enhance the signal strength in peroxidase (HRP) detections and to modify the signal colour from brown to purplish gray. Applications include all immunochemical assays like immunohistochemistry and -cytochemistry as well as Western blotting, but DAB metal enhancer may also well be used during in situ hybridization. DAB Metal Enhancer ready-to-use, for immunochemistry Mechanism When the DAB metal enhancer is applied, the sensitivity of DAB staining is about two times higher than with DAB alone. Figure: Immunohistochemical staining of mouse small intestines with an anti-PCNA antibody. Minuscules: pure DAB substrate, capitals: detection supplemented by DAB enhancer (A: NiCl 2 , B: DAB Metal Enhancer, Art. No. 9204). A/a: Basic method using 0.6 mg/ml DAB in Tris-buffer. B/b: Detection performed with the ROTI ® DAB Kit. Directions for use For application, the DAB substrate components and buffer reagents (for instance ROTI ® DAB-Kit, Art. No. 9202) are simply diluted in DAB metal enhancer instead of water. The purplish gray precipitate resulting from the staining reaction can be detected in visible light and does not bleach during long-term storage. Since it is insoluble in aqueous and organic solvents, slides may be permanently mounted in hydrophilic, or, after dehydration, in hydrophobic mounting medium. Figure 1: Dot blot of a HRP conjugated antibody (400, 200, 100, 50 and 25 pg). CR/cr: Detection using ROTI ® DAB Kit A-e: Detection using kits and DAB substrates from other suppliers CR: Detection under supplementation with DAB Metal Enhancer A: Detection under supplementation with NiCl 2
Product Detail
Buffer solution for preparation of antibody incubation solutions and stabile antibody dilutions for storage. With green dye for optimum handling or as a clear solution. Enhances signal strength Reduces background Very easy application Compatible with paraffin- and cryo sections Due to the particularly designed formulation, antigen/antibody binding is enhanced, while reducing background signals in parallel (Grube (2004) Arch Histol Cytol. 67:115-34 and Buchwalow et al. (2011) Sci Rep. 1:28). Stability of the antibodies is significantly enhanced. Directions for use The buffer solution is suitable for dilution of all primary and secondary antibodies, all mono- and polyclonals, as well as for all labelled as well as non-labelled antibodies. The antibodies are just diluted in the ready-to-use diluent and applied to the slides after blocking. For reduction of the amount needed and for optimised use of high-priced antibodies we recommend to use the ROTI ® Liquid Barrier Marker (red, Art. No. AN92, or colourless, Art. No. AN91), in order to prepare tiny incubation chambers on top of the slides. Antibody Diluent green ready-to-use, for immunohistochemistry Not a medical device / Not an IVD product
Product Detail
Kit for amplification of immunochemical detection signals on blot membranes and in ELISA. Strong enhancement of signal strength Most easy application The Signal Enhancer Western/ELISA enhances antigen-antibody reactions in a variety of immunoassays such as Western blotting, dot blotting and ELISA. It can significantly enhance detection of weak immunoreactive and low abundance target proteins and is well compatible with all common assay versions, membranes and ELISA plates. Signal enhancement is dependent on several parameters such as the particular antigen/antibody pair, the particular detection enzyme, the substrate chosen and so on. In most cases, however, the signal strength is about 5 to 10fold higher than with the substrate alone. Compatible with: Western and ELISA HRP and AP all common membranes all common substrates For application, the two solutions Signal Enhancer Western/ELISA-1 and -2 are simply used as buffer solutions for preparation of the primary- and secondary antibody incubation solutions. Following incubation and washing, all common detection procedures may be applied, for instance colour staining with ROTI ® DAB-Kit (Art. No. 9202) or chemoluminescent staining with ROTI ® Lumin plus (Art. No. 3692). Signal Enhancer Western/ELISA Set for immunochemistry Figure 1: Western blot. Detection of Rac1 in an extract from rat cortical primary neurons (1, 2 and 3 µg, Rac1 protein size 16 kDa) on PVDF. Detection via murine anti-Rac1 and anti-mouse-HRP. A: Base method w/o enhancer, B: Detection via Signal Enhancer Western/ELISA With kind permission of Dr. K. Ishizuka, Department of Psychiatry and Behavioural Sciences, Johns Hopkins University, Baltimore, USA The kit contains Signal Enhancer Western/ELISA-1 (Art. No. 9213) and Signal Enhancer Western/ELISA-2 (Art. No. 9214). 1 Mini-Set: 2 x 50 ml, 1 Set: 2 x 250 ml.
Product Detail
Kit for amplification of immunochemical detection signals on blot membranes and in ELISA. Strong enhancement of signal strength Most easy application The Signal Enhancer Western/ELISA enhances antigen-antibody reactions in a variety of immunoassays such as Western blotting, dot blotting and ELISA. It can significantly enhance detection of weak immunoreactive and low abundance target proteins and is well compatible with all common assay versions, membranes and ELISA plates. Signal enhancement is dependent on several parameters such as the particular antigen/antibody pair, the particular detection enzyme, the substrate chosen and so on. In most cases, however, the signal strength is about 5 to 10fold higher than with the substrate alone. Compatible with: Western and ELISA HRP and AP all common membranes all common substrates For application, the two solutions Signal Enhancer Western/ELISA-1 and -2 are simply used as buffer solutions for preparation of the primary- and secondary antibody incubation solutions. Following incubation and washing, all common detection procedures may be applied, for instance colour staining with ROTI ® DAB-Kit (Art. No. 9202) or chemoluminescent staining with ROTI ® Lumin plus (Art. No. 3692). Signal Enhancer Western/ELISA Set for immunochemistry Figure 1: Western blot. Detection of Rac1 in an extract from rat cortical primary neurons (1, 2 and 3 µg, Rac1 protein size 16 kDa) on PVDF. Detection via murine anti-Rac1 and anti-mouse-HRP. A: Base method w/o enhancer, B: Detection via Signal Enhancer Western/ELISA With kind permission of Dr. K. Ishizuka, Department of Psychiatry and Behavioural Sciences, Johns Hopkins University, Baltimore, USA The kit contains Signal Enhancer Western/ELISA-1 (Art. No. 9213) and Signal Enhancer Western/ELISA-2 (Art. No. 9214). 1 Mini-Set: 2 x 50 ml, 1 Set: 2 x 250 ml.
Product Detail
Buffer solution for preparation of antibody incubation solutions and stabile antibody dilutions for storage. With green dye for optimum handling or as a clear solution. Enhances signal strength Reduces background Very easy application Compatible with paraffin- and cryo sections Due to the particularly designed formulation, antigen/antibody binding is enhanced, while reducing background signals in parallel (Grube (2004) Arch Histol Cytol. 67:115-34 and Buchwalow et al. (2011) Sci Rep. 1:28). Stability of the antibodies is significantly enhanced. Directions for use The buffer solution is suitable for dilution of all primary and secondary antibodies, all mono- and polyclonals, as well as for all labelled as well as non-labelled antibodies. The antibodies are just diluted in the ready-to-use diluent and applied to the slides after blocking. For reduction of the amount needed and for optimised use of high-priced antibodies we recommend to use the ROTI ® Liquid Barrier Marker (red, Art. No. AN92, or colourless, Art. No. AN91), in order to prepare tiny incubation chambers on top of the slides. Antibody Diluent green ready-to-use, for immunohistochemistry Not a medical device / Not an IVD product
Product Detail
Buffer solution for preparation of antibody incubation solutions and stabile antibody dilutions for storage. With green dye for optimum handling or as a clear solution. Enhances signal strength Reduces background Very easy application Compatible with paraffin- and cryo sections Due to the particularly designed formulation, antigen/antibody binding is enhanced, while reducing background signals in parallel (Grube (2004) Arch Histol Cytol. 67:115-34 and Buchwalow et al. (2011) Sci Rep. 1:28). Stability of the antibodies is significantly enhanced. Directions for use The buffer solution is suitable for dilution of all primary and secondary antibodies, all mono- and polyclonals, as well as for all labelled as well as non-labelled antibodies. The antibodies are just diluted in the ready-to-use diluent and applied to the slides after blocking. For reduction of the amount needed and for optimised use of high-priced antibodies we recommend to use the ROTI ® Liquid Barrier Marker (red, Art. No. AN92, or colourless, Art. No. AN91), in order to prepare tiny incubation chambers on top of the slides. Antibody Diluent clear ready-to-use, for immunohistochemistry Not a medical device / Not an IVD product
Product Detail
Kit for amplification of immunochemical detection signals on blot membranes and in ELISA. Strong enhancement of signal strength Most easy application The Signal Enhancer Western/ELISA enhances antigen-antibody reactions in a variety of immunoassays such as Western blotting, dot blotting and ELISA. It can significantly enhance detection of weak immunoreactive and low abundance target proteins and is well compatible with all common assay versions, membranes and ELISA plates. Signal enhancement is dependent on several parameters such as the particular antigen/antibody pair, the particular detection enzyme, the substrate chosen and so on. In most cases, however, the signal strength is about 5 to 10fold higher than with the substrate alone. Compatible with: Western and ELISA HRP and AP all common membranes all common substrates For application, the two solutions Signal Enhancer Western/ELISA-1 and -2 are simply used as buffer solutions for preparation of the primary- and secondary antibody incubation solutions. Following incubation and washing, all common detection procedures may be applied, for instance colour staining with ROTI ® DAB-Kit (Art. No. 9202) or chemoluminescent staining with ROTI ® Lumin plus (Art. No. 3692). Signal Enhancer Western/ELISA-1 for immunochemistry The solution Signal Enhancer Western/ELISA-1 enhances reaction between antigens and their corresponding primary antibodies in a variety of immunoassays such as Western blotting, dot blotting and ELISA. Even for weak immunoreactive and low abundance target proteins strong signals may thus be achieved. The solution is well compatible with all common assay versions, membranes and ELISA plates. Directions for use For application, the solution Signal Enhancer Western/ELISA-1 is simply used as buffer solutions for preparation of the primary antibody incubation solution. Following incubation and washing, all common detection procedures may be applied, for instance colour staining with ROTI ® DAB-Kit (Art. No. 9202) or chemoluminescent staining with ROTI ® Lumin plus. When assays are performed using one antibody (labelled) only, application of Signal Enhancer Western/ELISA-2 is recommended.
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
