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3,3'-Diaminobenzidine tetrahydrochloride ≥98 %, p.a. Popular substrate of peroxidases. DAB is used for biochemical and histological detection of endogenous peroxidases like catalase and cytochome oxidase. Additionally, it is the most common substrate for horseradish peroxidase (HRP) in histochemical and cytological assays. DAB is oxidised by HRP, forming a brown precipitate, which is unsoluble in aqueous and organic solvents. This precipitate can be detected in visible light and does not bleach during long-term storage. Directions for use Working concentration: 0.05-0.1 % (0.5-1 mg/ml) DAB in buffer (PBS, TBS, pH 7.0-7.6) containing 0.01 % hydrogen peroxide. Always use freshly prepared working solution. For oxidation high concentrations of fresh hydrogen peroxide are needed.
Product Detail
DAB substrate kit for detection of peroxidase (HRP) activity on immunoblots, immunohisto- and cytochemical slides and during in situ hybridization. ROTI ® DAB Kit contains substrate, buffer solution and activation reagent, therefore including all three solutions necessary for HRP detection reaction. Application is very simple - all three kit solutions are diluted equally in water, and the blotting filter membrane, or the slides, are then incubated in this substrate solution. Since the solutions are delivered in drop dispensers, dosing is most convenient. ROTI ® DAB Kit for immunochemistry Mechanism DAB is oxidized by HRP, forming a brown precipitate, which is insoluble in aqueous and organic solvents. This precipitate can be detected in visible light and does not bleach during long-term storage. Figure: Immunohistochemical staining of mouse small intestines with an anti-PCNA antibody. Minuscules: pure DAB substrate, capitals: detection supplemented by DAB enhancer (A: NiCl 2 , B: DAB Metal Enhancer, Art. No. 9204). A/a: Basic method using 0.6 mg/ml DAB in Tris-buffer. B/b: Detection performed with the ROTI ® DAB Kit. Directions for use Addition of the DAB Metal Enhancer (Art. No. 9204) doubles the signal strength. Slides may be permanently mounted in hydrophilic, or, after dehydration, in hydrophobic mounting medium. The kit contains DAB solution (Art. No. 9869), DAB buffer (Art. No. 9870), and Activation reagent (Art. No. 9871) in dropper bottles. Contents of this Kit may not be bought separately.
Product Detail
3,3'-Diaminobenzidine tetrahydrochloride ≥98 %, p.a. Popular substrate of peroxidases. DAB is used for biochemical and histological detection of endogenous peroxidases like catalase and cytochome oxidase. Additionally, it is the most common substrate for horseradish peroxidase (HRP) in histochemical and cytological assays. DAB is oxidised by HRP, forming a brown precipitate, which is unsoluble in aqueous and organic solvents. This precipitate can be detected in visible light and does not bleach during long-term storage. Directions for use Working concentration: 0.05-0.1 % (0.5-1 mg/ml) DAB in buffer (PBS, TBS, pH 7.0-7.6) containing 0.01 % hydrogen peroxide. Always use freshly prepared working solution. For oxidation high concentrations of fresh hydrogen peroxide are needed.
Product Detail
3,3'-Diaminobenzidine tetrahydrochloride ≥98 %, p.a. Popular substrate of peroxidases. DAB is used for biochemical and histological detection of endogenous peroxidases like catalase and cytochome oxidase. Additionally, it is the most common substrate for horseradish peroxidase (HRP) in histochemical and cytological assays. DAB is oxidised by HRP, forming a brown precipitate, which is unsoluble in aqueous and organic solvents. This precipitate can be detected in visible light and does not bleach during long-term storage. Directions for use Working concentration: 0.05-0.1 % (0.5-1 mg/ml) DAB in buffer (PBS, TBS, pH 7.0-7.6) containing 0.01 % hydrogen peroxide. Always use freshly prepared working solution. For oxidation high concentrations of fresh hydrogen peroxide are needed.
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© 2026 Copyright. All Rights Reserved.
