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The TAE buffer is optimally suited as a gel and running buffer system. Mechanism TAE buffer has a lower buffer capacity than TBE and can be used with higher voltages, longer gels and larger DNA fragments. The gels heat up during running to a lesser extent than when using the running buffer TBE. DNA migrates in TAE gel about twice as fast as in TBE buffered gels. ROTIPHORESE ® 50x TAE Buffer ROTIPHORESE ® 50x conc. ROTIPHORESE ® 50x TAE Buffer contains 2 M Tris, 1 M acetic acid and 50 mM EDTA in distilled, deionised water; pH 8,5. Directions for use ROTIPHORESE ® TAE buffer is usually used as 1x concentrated solution. TAE buffer is suitable for the separation of DNA fragments of all sizes in gels. For the run of gels for gel extraction of DNA, we recommend using ROTIPHORESE ® 10x TAE-Puffer light.
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Concentrated stock solution for tris/glycine/SDS gel run buffer (PAGE) Standardised composition according to Lämmli ( Nature ) Made from high-quality reagents For constant quality and highly reproducible gels Mechanism SDS denatures proteins and binds in a constant mass ratio to their peptide chains, covering their intrinsic charge. In the collection gel (pH 6.8) all proteins are kept in a sharp band between the neutral Glycin and chloride ions and thus all reach the separation gel at the same time. After the pH change when the separation gel is reached (pH 8.8), the chloride ions and the now cationic Glycin migrate very quickly to the anode and leave the proteins to themselves, which now move in the electric field depending on size towards the anode. ROTIPHORESE ® 10x SDS-PAGE ROTIPHORESE ® 10x conc. ROTIPHORESE ® 10x SDS-PAGE contains 0,25 M Tris, 1,92 M glycine and 1 % (w/v) SDS in distilled, deionised water for protein analysis. Acc. to Laemmli (1970), Nature 227:680. Directions for use ROTIPHORESE ® 10x SDS-PAGE is usually used as 1x concentrated solution. SDS-PAGE buffer is suitable for the denaturing separation of proteins and peptides of any size in the discontinuous polyacrylamide gel (collection gel / separation gel).
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Concentrated stock solution for tris/glycine/SDS gel run buffer (PAGE) Standardised composition according to Lämmli ( Nature ) Made from high-quality reagents For constant quality and highly reproducible gels Mechanism SDS denatures proteins and binds in a constant mass ratio to their peptide chains, covering their intrinsic charge. In the collection gel (pH 6.8) all proteins are kept in a sharp band between the neutral Glycin and chloride ions and thus all reach the separation gel at the same time. After the pH change when the separation gel is reached (pH 8.8), the chloride ions and the now cationic Glycin migrate very quickly to the anode and leave the proteins to themselves, which now move in the electric field depending on size towards the anode. ROTIPHORESE ® 10x SDS-PAGE ROTIPHORESE ® 10x conc. ROTIPHORESE ® 10x SDS-PAGE contains 0,25 M Tris, 1,92 M glycine and 1 % (w/v) SDS in distilled, deionised water for protein analysis. Acc. to Laemmli (1970), Nature 227:680. Directions for use ROTIPHORESE ® 10x SDS-PAGE is usually used as 1x concentrated solution. SDS-PAGE buffer is suitable for the denaturing separation of proteins and peptides of any size in the discontinuous polyacrylamide gel (collection gel / separation gel).
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The TAE buffer is optimally suited as a gel and running buffer system. Mechanism TAE buffer has a lower buffer capacity than TBE and can be used with higher voltages, longer gels and larger DNA fragments. The gels heat up during running to a lesser extent than when using the running buffer TBE. DNA migrates in TAE gel about twice as fast as in TBE buffered gels. ROTIPHORESE ® 10x TAE Buffer light ROTIPHORESE ® 10x conc., reduced EDTA concentration ROTIPHORESE ® 10x TAE-buffer light is particularly ideal for agarose gels in DNA-elution. Although the reduced EDTA-content lowers the contamination of the eluted DNA with EDTA and therefore prevents any interference of enzymatic activity, e.g. from ligases, isomerases or polymerases, the buffer properties still remain totally intact. Particularly suitable for in-gel-applications when used with ROTI ® Garose Low Melt (Art. No. 6351). ROTIPHORESE ® 4 M Tris-acetate and 1 mM EDTA in distilled, deionised water; pH 8,3. Directions for use ROTIPHORESE ® TAE buffer light is usually used as 1x concentrated solution. TAE buffer is suitable for the separation of DNA fragments of all sizes in gels. Optimized for the run of gels for the gel extraction of DNA.
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The TBE buffer is optimally suited as a gel and running buffer system. Mechanism TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels. ROTIPHORESE ® TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels. ROTIPHORESE ® 10x TBE Buffer ROTIPHORESE ® 10x conc. ROTIPHORESE ® 10x TBE buffer contains 1,0 M Tris-Borate pH 8,3 and 20 mM EDTA in distilled, deionised water; pH 8,3. Suitable for manufacturing sequencing gels with ROTIPHORESE ® Gel 40 and as a running buffer. Directions for use ROTIPHORESE ® TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA.
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The TBE buffer is optimally suited as a gel and running buffer system. Mechanism TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels. ROTIPHORESE ® TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels. ROTIPHORESE ® 10x TBE Buffer ROTIPHORESE ® 10x conc. ROTIPHORESE ® 10x TBE buffer contains 1,0 M Tris-Borate pH 8,3 and 20 mM EDTA in distilled, deionised water; pH 8,3. Suitable for manufacturing sequencing gels with ROTIPHORESE ® Gel 40 and as a running buffer. Directions for use ROTIPHORESE ® TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA.
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The TBE buffer is optimally suited as a gel and running buffer system. Mechanism TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels. ROTIPHORESE ® TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels. ROTIPHORESE ® 10x TBE Buffer ROTIPHORESE ® 10x conc. ROTIPHORESE ® 10x TBE buffer contains 1,0 M Tris-Borate pH 8,3 and 20 mM EDTA in distilled, deionised water; pH 8,3. Suitable for manufacturing sequencing gels with ROTIPHORESE ® Gel 40 and as a running buffer. Directions for use ROTIPHORESE ® TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA.
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The TAE buffer is optimally suited as a gel and running buffer system. Mechanism TAE buffer has a lower buffer capacity than TBE and can be used with higher voltages, longer gels and larger DNA fragments. The gels heat up during running to a lesser extent than when using the running buffer TBE. DNA migrates in TAE gel about twice as fast as in TBE buffered gels. ROTIPHORESE ® 10x TAE Buffer ROTIPHORESE ® 10x conc. ROTIPHORESE ® 10x TAE Buffer contains 0,4 M Tris, 0,2 M acetic acid and 10 mM EDTA in distilled, deionised water; pH 8,3. Directions for use TAE buffer is usually used as 1x concentrated solution. TAE buffer is suitable for the separation of DNA fragments of all sizes in gels. For the run of gels for gel extraction of DNA, we recommend using ROTIPHORESE ® 10x TAE-Puffer light.
Product Detail
The TAE buffer is optimally suited as a gel and running buffer system. Mechanism TAE buffer has a lower buffer capacity than TBE and can be used with higher voltages, longer gels and larger DNA fragments. The gels heat up during running to a lesser extent than when using the running buffer TBE. DNA migrates in TAE gel about twice as fast as in TBE buffered gels. ROTIPHORESE ® 10x TAE Buffer ROTIPHORESE ® 10x conc. ROTIPHORESE ® 10x TAE Buffer contains 0,4 M Tris, 0,2 M acetic acid and 10 mM EDTA in distilled, deionised water; pH 8,3. Directions for use TAE buffer is usually used as 1x concentrated solution. TAE buffer is suitable for the separation of DNA fragments of all sizes in gels. For the run of gels for gel extraction of DNA, we recommend using ROTIPHORESE ® 10x TAE-Puffer light.
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The TBE buffer is optimally suited as a gel and running buffer system. Mechanism TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels. ROTIPHORESE ® TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels. ROTIPHORESE ® NF 10x TBE Buffer ROTIPHORESE ® 10x conc., fluorescence-free 10-times concentrated buffer formulated specially for automated sequencing. 10x stock solution contains 0,89 M Tris-Borate and 22 mM EDTA in distilled, deionised water; pH 8,3. Directions for use Suitable for manufacturing sequencing gels with ROTIPHORESE ® NF Acrylamide/Bis-solution 40 % (19:1) and as running buffer.In sequencing, NF-TBE buffer is usually used in a final concentration of 1x. Ideally suited as a gel and running buffer system and also for sequencing gels,
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The TBE buffer is optimally suited as a gel and running buffer system. Mechanism TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels. ROTIPHORESE ® TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels. ROTIPHORESE ® 5x TBE Buffer ROTIPHORESE ® 5x conc. ROTIPHORESE ® 5x TBE buffer contains 0,5 M Tris-Borate pH 8,3 and 10 mM EDTA in distilled, deionised water; pH 8,3. Suitable for manufacturing sequencing gels with ROTIPHORESE ® Gel 40 and as a running buffer. Directions for use ROTIPHORESE ® TBE buffer is usually used as 1x or 0.5-fold concentrated solution. TBE buffer is particularly suitable for the separation of small DNA fragments in the gel (up to 3 kb). TBE buffer is not suitable for use of gels for gel extraction of DNA. Mechanism TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.
Product Detail
Concentrated stock solution for tris/glycine/SDS gel run buffer (PAGE) Standardised composition according to Lämmli ( Nature ) Made from high-quality reagents For constant quality and highly reproducible gels Mechanism SDS denatures proteins and binds in a constant mass ratio to their peptide chains, covering their intrinsic charge. In the collection gel (pH 6.8) all proteins are kept in a sharp band between the neutral Glycin and chloride ions and thus all reach the separation gel at the same time. After the pH change when the separation gel is reached (pH 8.8), the chloride ions and the now cationic Glycin migrate very quickly to the anode and leave the proteins to themselves, which now move in the electric field depending on size towards the anode. ROTIPHORESE ® 10x SDS-PAGE ROTIPHORESE ® 10x conc. ROTIPHORESE ® 10x SDS-PAGE contains 0,25 M Tris, 1,92 M glycine and 1 % (w/v) SDS in distilled, deionised water for protein analysis. Acc. to Laemmli (1970), Nature 227:680. Directions for use ROTIPHORESE ® 10x SDS-PAGE is usually used as 1x concentrated solution. SDS-PAGE buffer is suitable for the denaturing separation of proteins and peptides of any size in the discontinuous polyacrylamide gel (collection gel / separation gel).
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
