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DNAse-, RNAse- and Protease-free Free of PCR inhibitors like modified bases and tetra-pyrophosphate Adjusted to pH 7,5 for optimzed enzyme compatibility Highly efficient enzymatic fabrication Can be optimally combined with Carl ROTH ROTI ® Pol DNA polymerases Suitable for All Carl ROTH nucleotides are manufactured from highest-quality reagents and are most thoroughly tested for quality. This testing procedure not only includes standard-PCR but also “long range PCR”, repeated quantitative light-cycling reactions, and tests for physical stability. Rhodamine-12-dUTP ≥95 %, 1 mM solution Rhodamine-12-dUTP is able to replace dTTP in growing DNA-strands and is used for efficient non-radioactive DNA-labelling. Detection of labelled nucleic acids can easily be done by direct analysis of fluorescent signals (e. g. by fluorescence microscopy). Rhodamine-12-dUTP can also be used for double staining techniques in combination with fluorescein-12-dUTP or biotin-11-dUTP and corresponding antibodies or streptavidin-complexes, respectively. Excitation: 505 nm Emission: 530 nm (red) Application examples Non-radioactive labelling of DNA by enzymatic reactions, e.g. PCR, reverse transcription, nick-end-translation, end-labelling or random-primed DNA-labelling. Incorporation can be done with all established DNA-polymerases (e.g. Taq-polymerase, T4 DNA-polymerase, Klenow fragment). Tested for the lack of endo-, exodeoxyribonuclease, ribonuclease and phosphatase. Rhodamine Green, mixture of 5/6 isomeres ε 505 (pH 7) = 8,5 E x mmol -1 x cm -1 ; pH: 7,5 ±0,2
Product Detail
DNAse-, RNAse- and Protease-free Free of PCR inhibitors like modified bases and tetra-pyrophosphate Adjusted to pH 7,5 for optimzed enzyme compatibility Highly efficient enzymatic fabrication Can be optimally combined with Carl ROTH ROTI ® Pol DNA polymerases Suitable for All Carl ROTH nucleotides are manufactured from highest-quality reagents and are most thoroughly tested for quality. This testing procedure not only includes standard-PCR but also “long range PCR”, repeated quantitative light-cycling reactions, and tests for physical stability. Rhodamine-12-dUTP ≥95 %, 1 mM solution Rhodamine-12-dUTP is able to replace dTTP in growing DNA-strands and is used for efficient non-radioactive DNA-labelling. Detection of labelled nucleic acids can easily be done by direct analysis of fluorescent signals (e. g. by fluorescence microscopy). Rhodamine-12-dUTP can also be used for double staining techniques in combination with fluorescein-12-dUTP or biotin-11-dUTP and corresponding antibodies or streptavidin-complexes, respectively. Excitation: 505 nm Emission: 530 nm (red) Application examples Non-radioactive labelling of DNA by enzymatic reactions, e.g. PCR, reverse transcription, nick-end-translation, end-labelling or random-primed DNA-labelling. Incorporation can be done with all established DNA-polymerases (e.g. Taq-polymerase, T4 DNA-polymerase, Klenow fragment). Tested for the lack of endo-, exodeoxyribonuclease, ribonuclease and phosphatase. Rhodamine Green, mixture of 5/6 isomeres ε 505 (pH 7) = 8,5 E x mmol -1 x cm -1 ; pH: 7,5 ±0,2
Product Detail© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
