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Sterile Minimum Essential Medium is a commonly used modification of the Basal Medium acc. to Eagle, having been developed in 1959 for culturing of HeLa cells. MEM contains high concentration of amino acids, which conform closely to the protein composition of human cells. Concentration of vitamins, and other essential nutrients, is enhanced, allowing longer cultivation periods between feedings. MEM is one of the most widely used cell culture media and can be used for a variety of suspension cell cultures and adherent cells. Generally, FBS is added to MEM basal media. ROTI ® Cell Eagle’s MEM / Earle’s CELLPURE ® ready-to-use, sterile, with stable glutamine, w/o pyruvate This modification of MEM contains stable glutamine and Earle’s salts, but no pyruvate. Stable glutamine is present as the dipeptide L-alanyl-L-glutamine. Due to its reinforced structure, this form of glutamine is not temperature sensitive, unlike normal L-glutamine, and therefore does not spontaneously decompose to toxic ammonia. Cells are able to break down this dipeptide bond and release L-glutamine as needed. Thus, the low ammonia concentration improves cell viability and growth. In addition, the concentration of L-glutamine is kept constant. Stable gluatmine has already been added to some cell culture media, but can also be purchased as a separate additive solution, the ROTI ® Cell Glutamine Solution.
Product Detail
Sterile Minimum Essential Medium is a commonly used modification of the Basal Medium acc. to Eagle, having been developed in 1959 for culturing of HeLa cells. MEM contains high concentration of amino acids, which conform closely to the protein composition of human cells. Concentration of vitamins, and other essential nutrients, is enhanced, allowing longer cultivation periods between feedings. MEM is one of the most widely used cell culture media and can be used for a variety of suspension cell cultures and adherent cells. Generally, FBS is added to MEM basal media. ROTI ® Cell Eagle’s MEM / Earle’s CELLPURE ® ready-to-use, sterile, w/o glutamine This modification of MEM contains Earle's salts, but no L-glutamine and no pyruvate.
Product Detail
Sterile Alpha-Eagle’s Minimum Essential Medium is based on EMEM, containing the following unique components: Vitamin B 12 , ascorbic acid, non-essential amino acids, sodium pyruvate, lipoic acid, and D-biotin. MEM-Alpha media can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. Generally, FBS is added to MEM-Alpha media. ROTI ® Cell Eagle’s MEM-Alpha CELLPURE ® ready-to-use, sterile, w/o glutamine, with pyruvate, with nucleosides This modification of DMEM contains nucleosides, pyruvate and Earle's salts but no glutamine.
Product Detail
Sterile Alpha-Eagle’s Minimum Essential Medium is based on EMEM, containing the following unique components: Vitamin B 12 , ascorbic acid, non-essential amino acids, sodium pyruvate, lipoic acid, and D-biotin. MEM-Alpha media can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. Generally, FBS is added to MEM-Alpha media. ROTI ® Cell Eagle’s MEM-Alpha CELLPURE ® ready-to-use, sterile, w/o glutamine, w/o nucleosides This modification of DMEM contains pyruvate and Earle's salts but no glutamine and nucleosides. MEM-Alpha media without nucleosides can be used for the selection of transfected DG44 and other AHFR-negative cells.
Product Detail
Sterile Minimum Essential Medium is a commonly used modification of the Basal Medium acc. to Eagle, having been developed in 1959 for culturing of HeLa cells. MEM contains high concentration of amino acids, which conform closely to the protein composition of human cells. Concentration of vitamins, and other essential nutrients, is enhanced, allowing longer cultivation periods between feedings. MEM is one of the most widely used cell culture media and can be used for a variety of suspension cell cultures and adherent cells. Generally, FBS is added to MEM basal media. ROTI ® Cell Eagle’s MEM / Earle’s CELLPURE ® ready-to-use, sterile, with glutamine This modification of MEM contains L-glutamine and Earle's salts, but no pyruvate.
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