For preparation of 4-20 % sequencing gels Most simple handling of three optimised ready-to-use solutions No tedious dissolving of urea Prevents acrylamide dusts Reproducible, superior quality of gels In tried-and-tested ROTIPHORESE ® quality Convenient set containing three ready-made solutions for easy and fast casting of sequencing gels. Solutions are produced in ROTIPHORESE ® quality and are perfectly matched - providing superior, reproducible quality and high sequence resolution. In addition, the ROTIPHORESE ® Sequencing system allows flexible adaptation to the desired gel variant, so that the amount of urea can be varied in addition to the acrylamide concentration. By using the ROTIPHORESE ® Sequencing gel buffer concentrate and ROTIPHORESE ® Sequencing gel diluent contained in the set, gels with 50 % urea content are obtained. By adding defined quantities of ROTIPHORESE ® 10x TBE buffer or water, the urea content can be reduced to up to 40 %. Directions for use Handling is most simple: Measure and mix certain amounts of all three components, add APS and TEMED, and cast the gel. We recommend using the Carl ROTH sequencing electrophoresis chamber (Art. no. AE28). ROTIPHORESE ® Sequencing gel concentrate ROTIPHORESE ® 25 % solution (19:1), ready-to-use, gas-stabilized Ready-to-use, stabilised solution of 25 % acrylamide/bisacrylamide 19:1. For the preparation of polyacrylamide sequencing gels containing 0-20 % monomer; 23,75 % acrylamide,1,25 % methylene bisacrylamide and 40-50 % urea (6,7-8,3 M). Cross linking 5. Also part of the ROTIPHORESE ® DNA sequencing system (Art. No. A431.1). Not a medical device / Not an IVD product
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