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The following plasmid DNA was prepared in low nuclease host bacteria and isolated through ion exchange chromatography. It is available in supercoiled form (ccc-form >95%) and, as a high-purity DNA (OD 260/280 > 1,70), it is ideal for different applications: Controlling transformation efficiency Comparison with plasmid ccc-forms of unknown size Cloning DNA-fragments Restriction (Preparing DNA-length markers) Calibrating DNA-separating or DNA-preparing processes or equipment Blocking DNA-chips with plasmid DNA of a defined sequence Plasmid DNA pUC19 lyophilized pUC19 is a standard high-copy cloning vector for E. coli recombinants. The position of the Multi Cloning Site (MCS), which is inserted in frame into the LacZ-gene, enables a blue-white selection of insert containing plasmid DNA by α-complementation. The copy number of plasmids per cell depends on the temperature and is approx. 70-80 at 37 °C and over 200 at 42 °C. General properties: OD 260/280 : >1,70 Genomic DNA: <2 % oc form: <3 % ccc form: >95 % Base pairs: 2686 Suitable for the blue / white selection
Product Detail
The following plasmid DNA was prepared in low nuclease host bacteria and isolated through ion exchange chromatography. It is available in supercoiled form (ccc-form >95%) and, as a high-purity DNA (OD 260/280 > 1,70), it is ideal for different applications: Controlling transformation efficiency Comparison with plasmid ccc-forms of unknown size Cloning DNA-fragments Restriction (Preparing DNA-length markers) Calibrating DNA-separating or DNA-preparing processes or equipment Blocking DNA-chips with plasmid DNA of a defined sequence Plasmid DNA pBR322 lyophilized The circular sequence was numbered in such a way that 0 lies in the middle of the singular EcoR l restriction site. The copy number of pBR322 is limited by the gene product of rop to 20 copies per cell. However, by adding chloroamphenicol (final concentration 170 µg/ml) to log-phase cultures and then incubating for 8 hours, the copy number can be increased considerably. General properties: OD 260/280 : >1,70 Genomic DNA: <2 % oc form: <3 % ccc form: >95 % Base pairs: 4361
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
