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Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol ROTI ® C/I ready-to-use, for the extraction of nucleic acids Mixture of chloroform and isoamyl alcohol of highest purity. Ideal for extraction of DNA/RNA in combination with ROTI ® Phenol, especially for the purification of nucleic acid containing solutions from residual phenol. Mechanism Addition of 1 volume ROTI ® C/I and thorough mixing. Chloroform dissolves residual phenol from the sample solution and leads to the denaturation of residual proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. Following centrifugation, the purified nucleic acid containing solution may be retrieved as upper phase.
Product Detail
Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol Mechanism Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl. Reduce exposure to toxic chemicals Prepared from phenol of highest purity Packed under argon for maximum stability Successfully tried and tested in many research laboratories ROTI ® Phenol/Chloroform/ Isoamyl alcohol ready-to-use, for the extraction of nucleic acids ROTI ® Phenol/Chloroform/Isoamyl alcohol is re-distilled phenol, equilibrated in TE buffer, premixed with high purity chloroform and isoamyl alcohol. ROTI ® Phenol/Chloroform/Isoamyl alcohol has proven itself in many research laboratories for the isolation of nucleic acids and is an ideal complement to pure ROTI ® Phenol. Mechanism Addition of 1 volume ROTI ® Phenol/Chloroform/Isoamyl alcohol and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. Following centrifugation, the nucleic acid containing solution may be retrieved as upper phase. Draw solution from lower phase. Do not shake before use!
Product Detail
Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol Mechanism Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl. Reduce exposure to toxic chemicals Prepared from phenol of highest purity Packed under argon for maximum stability Successfully tried and tested in many research laboratories ROTI ® Phenol ready-to-use, for the extraction of nucleic acids Phenol solutions for nucleic acid isolation must be buffered and adjusted to the correct pH value prior to use. ROTI ® Phenol is a redistilled, in TE buffer equilibrated readytouse phenol. ROTI ® Phenol has been successfully tried and tested for isolating nucleic acids in many research laboratories. This quality product for molecular biology replaces the time consuming preparation of phenol and subsequently any exposure to toxic vapours. Draw solution from lower phase. Do not shake before use! Mechanism Addition of 1 volume ROTI ® Phenol and thorough mixing. Phenol leads to denaturation of proteins which accumulate in the interphase. Following centrifugation, the nucleic acid containing solution may be retrieved as upper phase.
Product Detail
Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol ROTI ® C/I ready-to-use, for the extraction of nucleic acids Mixture of chloroform and isoamyl alcohol of highest purity. Ideal for extraction of DNA/RNA in combination with ROTI ® Phenol, especially for the purification of nucleic acid containing solutions from residual phenol. Mechanism Addition of 1 volume ROTI ® C/I and thorough mixing. Chloroform dissolves residual phenol from the sample solution and leads to the denaturation of residual proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. Following centrifugation, the purified nucleic acid containing solution may be retrieved as upper phase.
Product Detail
Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol Mechanism Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl. Reduce exposure to toxic chemicals Prepared from phenol of highest purity Packed under argon for maximum stability Successfully tried and tested in many research laboratories ROTI ® Phenol ready-to-use, for the extraction of nucleic acids Phenol solutions for nucleic acid isolation must be buffered and adjusted to the correct pH value prior to use. ROTI ® Phenol is a redistilled, in TE buffer equilibrated readytouse phenol. ROTI ® Phenol has been successfully tried and tested for isolating nucleic acids in many research laboratories. This quality product for molecular biology replaces the time consuming preparation of phenol and subsequently any exposure to toxic vapours. Draw solution from lower phase. Do not shake before use! Mechanism Addition of 1 volume ROTI ® Phenol and thorough mixing. Phenol leads to denaturation of proteins which accumulate in the interphase. Following centrifugation, the nucleic acid containing solution may be retrieved as upper phase.
Product Detail
Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol Mechanism Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl. Reduce exposure to toxic chemicals Prepared from phenol of highest purity Packed under argon for maximum stability Successfully tried and tested in many research laboratories ROTI ® Phenol ready-to-use, for the extraction of nucleic acids Phenol solutions for nucleic acid isolation must be buffered and adjusted to the correct pH value prior to use. ROTI ® Phenol is a redistilled, in TE buffer equilibrated readytouse phenol. ROTI ® Phenol has been successfully tried and tested for isolating nucleic acids in many research laboratories. This quality product for molecular biology replaces the time consuming preparation of phenol and subsequently any exposure to toxic vapours. Draw solution from lower phase. Do not shake before use! Mechanism Addition of 1 volume ROTI ® Phenol and thorough mixing. Phenol leads to denaturation of proteins which accumulate in the interphase. Following centrifugation, the nucleic acid containing solution may be retrieved as upper phase.
Product Detail
Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol Mechanism Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl. Reduce exposure to toxic chemicals Prepared from phenol of highest purity Packed under argon for maximum stability Successfully tried and tested in many research laboratories ROTI ® Phenol/Chloroform/ Isoamyl alcohol ready-to-use, for the extraction of nucleic acids ROTI ® Phenol/Chloroform/Isoamyl alcohol is re-distilled phenol, equilibrated in TE buffer, premixed with high purity chloroform and isoamyl alcohol. ROTI ® Phenol/Chloroform/Isoamyl alcohol has proven itself in many research laboratories for the isolation of nucleic acids and is an ideal complement to pure ROTI ® Phenol. Mechanism Addition of 1 volume ROTI ® Phenol/Chloroform/Isoamyl alcohol and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. Following centrifugation, the nucleic acid containing solution may be retrieved as upper phase. Draw solution from lower phase. Do not shake before use!
Product Detail
Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol ROTI ® C/I ready-to-use, for the extraction of nucleic acids Mixture of chloroform and isoamyl alcohol of highest purity. Ideal for extraction of DNA/RNA in combination with ROTI ® Phenol, especially for the purification of nucleic acid containing solutions from residual phenol. Mechanism Addition of 1 volume ROTI ® C/I and thorough mixing. Chloroform dissolves residual phenol from the sample solution and leads to the denaturation of residual proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. Following centrifugation, the purified nucleic acid containing solution may be retrieved as upper phase.
Product Detail
Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol Mechanism Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl. Reduce exposure to toxic chemicals Prepared from phenol of highest purity Packed under argon for maximum stability Successfully tried and tested in many research laboratories ROTI ® Phenol ready-to-use, for the extraction of nucleic acids Phenol solutions for nucleic acid isolation must be buffered and adjusted to the correct pH value prior to use. ROTI ® Phenol is a redistilled, in TE buffer equilibrated readytouse phenol. ROTI ® Phenol has been successfully tried and tested for isolating nucleic acids in many research laboratories. This quality product for molecular biology replaces the time consuming preparation of phenol and subsequently any exposure to toxic vapours. Draw solution from lower phase. Do not shake before use! Mechanism Addition of 1 volume ROTI ® Phenol and thorough mixing. Phenol leads to denaturation of proteins which accumulate in the interphase. Following centrifugation, the nucleic acid containing solution may be retrieved as upper phase.
Product Detail
Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol Mechanism Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl. Reduce exposure to toxic chemicals Prepared from phenol of highest purity Packed under argon for maximum stability Successfully tried and tested in many research laboratories ROTI ® Phenol/Chloroform/ Isoamyl alcohol ready-to-use, for the extraction of nucleic acids ROTI ® Phenol/Chloroform/Isoamyl alcohol is re-distilled phenol, equilibrated in TE buffer, premixed with high purity chloroform and isoamyl alcohol. ROTI ® Phenol/Chloroform/Isoamyl alcohol has proven itself in many research laboratories for the isolation of nucleic acids and is an ideal complement to pure ROTI ® Phenol. Mechanism Addition of 1 volume ROTI ® Phenol/Chloroform/Isoamyl alcohol and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. Following centrifugation, the nucleic acid containing solution may be retrieved as upper phase. Draw solution from lower phase. Do not shake before use!
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
