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ROTI ® Quick kit ready-to-use, for molecular biology Column-free, ultra-flexible isolation system designed for reliable isolation of total RNA from almost any tissue. The ROTI ® Quick Kit is suitable for use with cell culture material, biopsies, high-fat or high-protein tissue, blood (Üçeyler et al. Neurology (2007) 69:42-9), solid plant material, mitochondria (Backert et al. Plant Molecular Biology (1997) 33:1037-50), mycoplasms (Weiner et al. Nucleic Acids Res. (2000) 28:4488-96) and many more. The kit can be used for both fresh tissue and frozen material. The ROTI ® Quick Kit is based on the GITC isolation method by Chomczynski and Sacchi (Chomczynski and Sacchi. (1987) Anal. Biochem. 162:156) and includes three optimised ready-to-use solutions that were developed by molecular biologists and have proven effective for many years. After the tissue has been homogenised, the RNA is purified in a single step and isolated by precipitation. Each isolation takes approximately 2,5 hours, and multiple isolations can easily be performed simultaneously. The incubation and centrifuging steps take about 2 hours. Easy, most versatile application High recovery rate of intact, very pure total RNA Column-free two-step isolation Applicable on nearly any tissue type Preparation as given in the instruction-for-use is optimised for RNA isolation from approx. 0,2 g of tissue or cell material. The amount of material isolated per batch may easily be up- or downscaled to larger or smaller amounts of tissue. The isolated total RNA is highly pure and may be used directly for all common down-stream applications, such as RT-PCR, Northern blotting and reverse transcription. Detailed instructions-for-use are enclosed with each kit. The kit contains: ROTI ® Quick Kit 1, ROTI ® Quick Kit 2 and ROTI ® Quick Kit 3. Contents of this Kit may not be bought separately.
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Sterile Water BioScience Grade, DEPC treated, sterile, nuclease-free, autoclaved Distilled water mixed with DEPC and steam sterilised. By DEPC Histidin residues in proteins are modified to N -carbethoxy histidin, resulting in inhibition of RNases and DNases. DEPC decomposes to CO 2 and ethanol during sterilisation. Also available as 50 reaction tubes or as 50 glass ampoules with 1 ml each nuclease free water in a tube rack. To prevent loss of volume in the 1 mltubes (T143.4), we recommend freezing the product at -20°C after receipt. After autoclaving, each lot is photometrically tested for optimal purity in the wave length range relevant for nucleic acid research.
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Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol Mechanism Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl. Reduce exposure to toxic chemicals Prepared from phenol of highest purity Packed under argon for maximum stability Successfully tried and tested in many research laboratories ROTI ® Aqua-Phenol ready-to-use, for RNA extraction ROTI ® Aqua Phenol is perfectly suited for RNA isolation and other procedures, requiring a low phenol pH value. The low pH results in interphase enrichment of the DNA, reducing DNA contamination in the RNA eluate. Also ideal for self-adjustment of the pH-value. If required, buffer solution (e.g. TE buffer) may be added directly into the bottle, as ROTI ® Aqua-Phenol comes in the following bottle sizes: A980.2: 100 ml in a 250 ml bottle A980.1: 250 ml in a 500 ml bottle A980.3: 500 ml in a 1 l bottle Mechanism Addition of 1 volume ROTI ® Aqua-Phenol and thorough mixing. Phenol leads to denaturation of proteins which accumulate in the interphase. The acid pH-value causes a depletion of the DNA, so that only little contaminating DNA is isolated. Following centrifugation, the RNA containing solution may be retrieved as upper phase. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 12 drops of 1 N HCl. Draw solution from lower phase. Do not shake before use!
Product Detail
Sterile Water BioScience Grade, DEPC treated, sterile, nuclease-free, autoclaved Distilled water mixed with DEPC and steam sterilised. By DEPC Histidin residues in proteins are modified to N -carbethoxy histidin, resulting in inhibition of RNases and DNases. DEPC decomposes to CO 2 and ethanol during sterilisation. Also available as 50 reaction tubes or as 50 glass ampoules with 1 ml each nuclease free water in a tube rack. To prevent loss of volume in the 1 mltubes (T143.4), we recommend freezing the product at -20°C after receipt. After autoclaving, each lot is photometrically tested for optimal purity in the wave length range relevant for nucleic acid research.
Product Detail
Sterile Water BioScience Grade, DEPC treated, sterile, nuclease-free, autoclaved Distilled water mixed with DEPC and steam sterilised. By DEPC Histidin residues in proteins are modified to N -carbethoxy histidin, resulting in inhibition of RNases and DNases. DEPC decomposes to CO 2 and ethanol during sterilisation. Also available as 50 reaction tubes or as 50 glass ampoules with 1 ml each nuclease free water in a tube rack. To prevent loss of volume in the 1 mltubes (T143.4), we recommend freezing the product at -20°C after receipt. After autoclaving, each lot is photometrically tested for optimal purity in the wave length range relevant for nucleic acid research.
Product Detail
Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol Mechanism Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl. Reduce exposure to toxic chemicals Prepared from phenol of highest purity Packed under argon for maximum stability Successfully tried and tested in many research laboratories ROTI ® Aqua-P/C/I ready-to-use, for RNA extraction ROTI ® Aqua-P/C/I is the optimal choice for RNA isolation and other procedures where a low phenol pH value is desired. The low pH results in interphase enrichment of the DNA, reducing DNA contamination in the RNA eluate. Mechanism Addition of 1 volume ROTI ® Aqua-P/C/I and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. The acid pH-value causes a depletion of the DNA, so that only little contaminating DNA is isolated. Following centrifugation, the RNA containing solution may be retrieved as upper phase. For distinct phase separation, ROTI ® Aqua-P/C/I needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 12 drops of 1 N HCl.
Product Detail
Sterile Water BioScience Grade, DEPC treated, sterile, nuclease-free, autoclaved Distilled water mixed with DEPC and steam sterilised. By DEPC Histidin residues in proteins are modified to N -carbethoxy histidin, resulting in inhibition of RNases and DNases. DEPC decomposes to CO 2 and ethanol during sterilisation. Also available as 50 reaction tubes or as 50 glass ampoules with 1 ml each nuclease free water in a tube rack. To prevent loss of volume in the 1 mltubes (T143.4), we recommend freezing the product at -20°C after receipt. After autoclaving, each lot is photometrically tested for optimal purity in the wave length range relevant for nucleic acid research.
Product Detail
ROTI ® ZOL RNA ready-to-use, for molecular biology ROTI ® ZOL RNA is a ready-to-use, green-coloured solution for isolating total RNA from biological samples such as cells or tissue. ROTI ® ZOL RNA is based on the phenolic isolation procedure known from Chomczynski and Sacchi ( Anal. Biochem. (1987) 162:156-9). Due to the advanced and optimized formulation, ROTI ® ZOL RNA results in very high recovery rates of highly pure total RNA. Application is very convenient and preparation of several samples in parallel is finished in less than one hour. Additionally, the green colour significantly simplifies the separation of the RNA containing aqueous phase, the DNA containig interphase, and the protein containing phenolic phase during the process. Easy and very rapid two-step RNA isolation High recovery rates of intact, very pure total RNA Applicable on a broad range of tissues and on cell types The purified RNA is ready for use in standard downstream applications such as RT-PCR Typical RNA yield from 5x10 6 cells: 30-35 μg Typical purity of RNA prepared from 5x10 6 cells: 2,08 (A 260 /A 280 ) Figures 1 and 2: Isolation of totalRNA by ROTI ® Zol RNA. Left: Tube after centrifugation. A: Aqueous phase - RNA containig, P: Phenolic phase - protein containing. DNA accumulates in the A/P interphase. Right: Typical gel of total RNA isolated by ROTI ® Zol RNA (extracted from 5x10 6 HL-60 cells).
Product Detail
Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol Mechanism Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl. Reduce exposure to toxic chemicals Prepared from phenol of highest purity Packed under argon for maximum stability Successfully tried and tested in many research laboratories ROTI ® Aqua-P/C/I ready-to-use, for RNA extraction ROTI ® Aqua-P/C/I is the optimal choice for RNA isolation and other procedures where a low phenol pH value is desired. The low pH results in interphase enrichment of the DNA, reducing DNA contamination in the RNA eluate. Mechanism Addition of 1 volume ROTI ® Aqua-P/C/I and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. The acid pH-value causes a depletion of the DNA, so that only little contaminating DNA is isolated. Following centrifugation, the RNA containing solution may be retrieved as upper phase. For distinct phase separation, ROTI ® Aqua-P/C/I needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 12 drops of 1 N HCl.
Product Detail
Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol Mechanism Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl. Reduce exposure to toxic chemicals Prepared from phenol of highest purity Packed under argon for maximum stability Successfully tried and tested in many research laboratories ROTI ® Aqua-P/C/I ready-to-use, for RNA extraction ROTI ® Aqua-P/C/I is the optimal choice for RNA isolation and other procedures where a low phenol pH value is desired. The low pH results in interphase enrichment of the DNA, reducing DNA contamination in the RNA eluate. Mechanism Addition of 1 volume ROTI ® Aqua-P/C/I and thorough mixing. Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. The acid pH-value causes a depletion of the DNA, so that only little contaminating DNA is isolated. Following centrifugation, the RNA containing solution may be retrieved as upper phase. For distinct phase separation, ROTI ® Aqua-P/C/I needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 12 drops of 1 N HCl.
Product Detail
ROTI ® ZOL RNA ready-to-use, for molecular biology ROTI ® ZOL RNA is a ready-to-use, green-coloured solution for isolating total RNA from biological samples such as cells or tissue. ROTI ® ZOL RNA is based on the phenolic isolation procedure known from Chomczynski and Sacchi ( Anal. Biochem. (1987) 162:156-9). Due to the advanced and optimized formulation, ROTI ® ZOL RNA results in very high recovery rates of highly pure total RNA. Application is very convenient and preparation of several samples in parallel is finished in less than one hour. Additionally, the green colour significantly simplifies the separation of the RNA containing aqueous phase, the DNA containig interphase, and the protein containing phenolic phase during the process. Easy and very rapid two-step RNA isolation High recovery rates of intact, very pure total RNA Applicable on a broad range of tissues and on cell types The purified RNA is ready for use in standard downstream applications such as RT-PCR Typical RNA yield from 5x10 6 cells: 30-35 μg Typical purity of RNA prepared from 5x10 6 cells: 2,08 (A 260 /A 280 ) Figures 1 and 2: Isolation of totalRNA by ROTI ® Zol RNA. Left: Tube after centrifugation. A: Aqueous phase - RNA containig, P: Phenolic phase - protein containing. DNA accumulates in the A/P interphase. Right: Typical gel of total RNA isolated by ROTI ® Zol RNA (extracted from 5x10 6 HL-60 cells).
Product Detail
Please note our Technical Information Brochure on the Internet next to the products: Phenolic DNA Isolation Background and protocol Mechanism Phenol and chloroform lead to denaturation of proteins which accumulate in the interphase. Isoamyl alcohol prevents foaming and very efficiently inhibits RNAses. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 1-2 drops of 1 N HCl. Reduce exposure to toxic chemicals Prepared from phenol of highest purity Packed under argon for maximum stability Successfully tried and tested in many research laboratories ROTI ® Aqua-Phenol ready-to-use, for RNA extraction ROTI ® Aqua Phenol is perfectly suited for RNA isolation and other procedures, requiring a low phenol pH value. The low pH results in interphase enrichment of the DNA, reducing DNA contamination in the RNA eluate. Also ideal for self-adjustment of the pH-value. If required, buffer solution (e.g. TE buffer) may be added directly into the bottle, as ROTI ® Aqua-Phenol comes in the following bottle sizes: A980.2: 100 ml in a 250 ml bottle A980.1: 250 ml in a 500 ml bottle A980.3: 500 ml in a 1 l bottle Mechanism Addition of 1 volume ROTI ® Aqua-Phenol and thorough mixing. Phenol leads to denaturation of proteins which accumulate in the interphase. The acid pH-value causes a depletion of the DNA, so that only little contaminating DNA is isolated. Following centrifugation, the RNA containing solution may be retrieved as upper phase. For distinct phase separation, ROTI ® Aqua-Phenol needs a pH of ≤4.0 in the aqueous upper phase. If needed, the upper phase may be acidified by adding 12 drops of 1 N HCl. Draw solution from lower phase. Do not shake before use!
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
