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ROTI ® Blue 5x conc. ROTI ® Blue is a colloidal Coomassie ® brilliant blue G250 staining agent for proteins in polyacrylamid gels. Its colloidal features enable the staining agent to be bound to the proteins with high specificity and only minimally to the gel matrix. There is no longer any need for the extensive washing steps for background decoloring. Most simple application No destaining necessary 5x more sensitive than Coomassie ® R 250 Figures: A: Horizontal IEF for determination of wheat-varieties. With kind permission of R. Knoblauch, LTZ Referat 23, Karlsruhe B: SDS-PAGE ROTI ® Mark Standard. ROTI ® Blue is approximately 5 x more sensitive than standard Coomassie ® staining solutions. Staining time takes 2 to 12 hours depending on the desired band intensity. Due to the fact that proteins may be stained without fixing the gel, membrane transfer and gel elution are still possible following staining. Particularly well suited for staining prior to MS-analyses (e. g. MALDI) and prior to gel elution.
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ROTI ® Black P for electrophoresis ROTI ® Black P is a kit for speedy, reproducible silver staining of proteins in free and foil-supported gels. Sensitive silver staining Transparent gel Reliable results ROTI ® Black P allows the detection of proteins from 1 ng/mm 2 due to its high sensitivity in combination with the very low background of the stained gels. Free gels can be stained without methanol, for carrier-supported gels methanol is required. The ROTI ® Black P Kit comes with an easy to follow protocol that guarantees you reliable results. The kit contains Sodium thiosulphate (Art. No. L534), silver nitrate (Art. No. L535), empty test tubes (Art. No. L536), formaldehyde (Art. No. L537), and sodium carbonate (Art. No. L538). Contents of this Kit may not be bought separately. Shipped without racket.
Product Detail
ROTI ® Black P for electrophoresis ROTI ® Black P is a kit for speedy, reproducible silver staining of proteins in free and foil-supported gels. Sensitive silver staining Transparent gel Reliable results ROTI ® Black P allows the detection of proteins from 1 ng/mm 2 due to its high sensitivity in combination with the very low background of the stained gels. Free gels can be stained without methanol, for carrier-supported gels methanol is required. The ROTI ® Black P Kit comes with an easy to follow protocol that guarantees you reliable results. The kit contains Sodium thiosulphate (Art. No. L534), silver nitrate (Art. No. L535), empty test tubes (Art. No. L536), formaldehyde (Art. No. L537), and sodium carbonate (Art. No. L538). Contents of this Kit may not be bought separately. Shipped without racket.
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Brilliant blue G 250 (C.I. 42655) for electrophoresis and biochemistry
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ROTI ® Blue quick 1x conc., ready-to-use ROTI ® Blue quick provides a new, very fast staining method for proteins in polyacrylamide gels. Due to the modern formulation, the dye binds highly specifically to proteins, but not to the gel matrix, hence eliminating the need to destain the gel. First bands are clearly visible within 3 minutes! Particularly fast, specific staining First bands are visible within 3 mins. No background - no destaining necessary Very simple application without heating, methanol, or acetic acid Stabilised for a longer shelf life Figure: SDS-PAGE with 3 µl Roti ® -Mark Standard and samples. Staining duration: 20 mins. Directions for use The following protein amounts / band can be detected in the specified time (minutes): from 1 µg (3 min.) from 100 µg (15 min.) from 10 ng (60 min.) The sensitivity of staining equals that of ROTI ® Blue (Art. No. A152), so bands of 10 ng protein can be detected. ROTI ® Blue quick is, therefore, approx. 5times more sensitive than standard Coomassie ® staining solutions. ROTI ® Blue quick staining is also fully compatible with usual down-stream applications like, membrane transfer & Western blotting, MS analysis (incl. MALDI) and protein gel elution.
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ROTI ® Blue quick 1x conc., ready-to-use ROTI ® Blue quick provides a new, very fast staining method for proteins in polyacrylamide gels. Due to the modern formulation, the dye binds highly specifically to proteins, but not to the gel matrix, hence eliminating the need to destain the gel. First bands are clearly visible within 3 minutes! Particularly fast, specific staining First bands are visible within 3 mins. No background - no destaining necessary Very simple application without heating, methanol, or acetic acid Stabilised for a longer shelf life Figure: SDS-PAGE with 3 µl Roti ® -Mark Standard and samples. Staining duration: 20 mins. Directions for use The following protein amounts / band can be detected in the specified time (minutes): from 1 µg (3 min.) from 100 µg (15 min.) from 10 ng (60 min.) The sensitivity of staining equals that of ROTI ® Blue (Art. No. A152), so bands of 10 ng protein can be detected. ROTI ® Blue quick is, therefore, approx. 5times more sensitive than standard Coomassie ® staining solutions. ROTI ® Blue quick staining is also fully compatible with usual down-stream applications like, membrane transfer & Western blotting, MS analysis (incl. MALDI) and protein gel elution.
Product Detail
Brilliant blue G 250 (C.I. 42655) for electrophoresis and biochemistry
Product Detail
ROTI ® Blue 5x conc. ROTI ® Blue is a colloidal Coomassie ® brilliant blue G250 staining agent for proteins in polyacrylamid gels. Its colloidal features enable the staining agent to be bound to the proteins with high specificity and only minimally to the gel matrix. There is no longer any need for the extensive washing steps for background decoloring. Most simple application No destaining necessary 5x more sensitive than Coomassie ® R 250 Figures: A: Horizontal IEF for determination of wheat-varieties. With kind permission of R. Knoblauch, LTZ Referat 23, Karlsruhe B: SDS-PAGE ROTI ® Mark Standard. ROTI ® Blue is approximately 5 x more sensitive than standard Coomassie ® staining solutions. Staining time takes 2 to 12 hours depending on the desired band intensity. Due to the fact that proteins may be stained without fixing the gel, membrane transfer and gel elution are still possible following staining. Particularly well suited for staining prior to MS-analyses (e. g. MALDI) and prior to gel elution.
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Silver nitrate Proteicolor ® ≥99,9 % PROTEICOLOR ® is a special quality silver nitrate for reproducible staining of proteins and 1,2 Diol-groups, of glycoproteins and polysaccharides in polyacrylamide and agarose gels. Staining with PROTEICOLOR ® can also be used for highly sensitive identification of RNA and DNA.
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ROTIPHORESE ® Blue R ROTIPHORESE ® concentrate Concentrate for easy preparation of Coomassie ® Blue R staining solutions. ROTIPHORESE ® Blue R is ideal for staining proteins in SDS-PAGE gels. It saves any tedious weighing of staining powder and having to prepare the mixture. Figure: Gel stained by ROTIPHORESE ® Blue R, 12% PAA in Tris, ROTI ® Mark STANDARD (Art. No. T851). Directions for use Concentrate contains Coomassie ® Blue R, methanol and pure acetic acid. ROTIPHORESE ® Blue R can be diluted directly in the bottle with 400 to 600 ml deionized water, resulting in the following composition depending on the dilution. When diluted with 397,5 ml water (to a total of 1 litre staining solution): Methanol (50 %), acetic acid 10 %, Coomassie ® R (0,25 %) When diluted with 507,5 ml water (to a total of 1,1 litre staining solution): Methanol (45 %), acetic acid 9 %, Coomassie ® R (0,22 %) When diluted with 602,5 ml water (to a total of 1,2 litre staining solution): Methanol (41,5 %), acetic acid 8,3 %, Coomassie ® R (0,2 %). We recommend mixing the concentrated solution well before dilution and filtering the diluted solution before application.
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© 2023 Copyright. All Rights Reserved. Unit 19, Euroway House, Roydsdale Way, Bradford, BD4 6SE, UK
